DFF40 Antibody

DFF40 Antibody

Catalog# : 2107

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease) (1,2). The human homologue of mouse CAD was more recently identified by three groups independently and termed CPAN, DFF40, and human CAD, respectively, (3-5). DFF45/ICAD is the inhibitory protein of DFF40/CAD (1,2,6) and forms complex with DFF40/CAD. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of DFF40/CAD, which causes DNA degradation, is the hallmark of apoptotic cell death.

Additional Names : DFF40, DFF40 (I18), CAD (I18)

Description
Left: Western blot analysis of DFF40 in HeLa (H), K562 (K), Jurkat (J), and Raji (R) whole cell lysate with DFF40 antibody (I18) at 1:500 dilution.

Below: Immunocytochemistry of DFF40 in Jurkat cells with DFF40 antibody at 5 µg/ml.

Other Product Images


Source : DFF40 antibody was raised against a peptide corresponding to amino acids near the center of murine CAD. The sequence differs from human DFF40 by two amino acids (3,4).

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : DFF40 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Immunogen : Human DFF40 Peptide (Cat. No. 2107P)

Application : DFF40 antibody can be used for detection of DFF40 by Western blot at 1:500 to 1:1000 dilution. A 40 kDa band can be detected.

Tested Application(s) : E, WB, ICC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 2107P - DFF40 Peptide

Long-Term Storage : DFF40 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1.Cat. No. 1204 - K562 Cell Lysate
2.Cat. No. 1205 Jurkat Whole Cell Lysate

Species Reactivity :H, M, R

GI Number : 160948620

Accession Number : NP_031885

Short Description : Caspase Activated DNase

References
1.Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50
2.Sakahira H, Enari M, Nagata S. Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis. Nature 1998;391:96-99
3.Halenbeck R, MacDonald H, Roulston A, Chen TT, Conroy L, Williams LT. CPAN, a human nuclease regulated by the caspase-sensitive inhibitor DFF45. Curr Biol 1998;8:537-40
4.Liu X, Li P, Widlak P, Zou H, Luo X, Garrard WT, Wang X The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis. Proc Natl Acad Sci USA 1998;95:8461-6