DRAK2 Antibody

DRAK2 Antibody

Catalog# : 2149

Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as ASK-1 and RIP, are mediators of apoptosis. Two novel serine/threonine kinases that induce apoptosis were recently identified and designated DRAK1 and DRAK2 (for DAP kinase-related apoptosis-inducing protein kinases) (1). DRAKs contain an N-terminal kinase domain and a C-terminal regulation domain. Overexpression of DRAK2 induces apoptosis. DRAKs have high sequence homology to DAP and ZIP kinases, and they represent a novel family of serine/threonine kinases, which mediates apoptosis through their catalytic activities. DRAK2 is located in nucleus and the messenger RNA was ubiquitously expressed in human tissues (1).

Additional Names : DRAK2 (CT), DRAK2
Description
Left: Western blot analysis of DRAK2 in Jurkat (1,3) and Raji (2,4) cell lysate in the absence (1,2) or presence (3,4) of blocking peptide with DRAK2 antibody at 1:500 dilution.

Below: Immunocytochemistry of DRAK2 in Jurkat cells with DRAK2 antibody at 10 µg/ml.

Other Product Images

Source : DRAK2 antibody was raised against a peptide corresponding to amino acids 351 to 365 of human DRAK2.

Purification : Ion exchange chromatography purified

Clonality and Clone : This is a polyclonal antibody.

Host : DRAK2 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Immunogen : Human DRAK2 (C-Terminus) Peptide (Cat. No. 2149P)

Application : DRAK2 antibody can be used for detection of DRAK2 by Western blot at 1:500 to 1:1000 dilution. An approximately 45 kDa band can be detected. It has no cross responses to DAP or ZIP kinases. The approximately 70 kDa band is probably non-related to DRAK2 although it is peptide blockable.

Tested Application(s) : E, WB, ICC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 2149P - DRAK2 Peptide

Long-Term Storage : DRAK2 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1.Cat. No. 1205 - Jurkat Cell Lysate

Species Reactivity :H

GI Number : 3834355

Accession Number : AB011421

Short Description : (CT) DAPK Related Apoptosis-inducing Kinase

References

1.Sanjo H, Kawai T, Akira S. DRAKs, novel serine/threonine kinases related to death-associated protein kinase that trigger apoptosis. J Biol Chem 1998;273:29066-71 (RD1299)