Taq Polyermase (10X Taq Reaction Buffer Mg-Free and 25 mM MgCl2)

Taq Polyermase (10X Taq Reaction Buffer Mg-Free and 25 mM MgCl2)

Alternate Name:
Catalog# : NB-28087
Price/Unit : $213.00
Unit : 1000 units (5 U/ul)

• Recombinant source
• Functionality preserved at wide range of temperatures
• Highly reliable and reproducible reactions
• Supplied with detergent-free, Mg-free Reaction Buffer for compatibility with microarray and DHPL applications and reactions that require adjustment of magnesium concentrations
• Compatible with the use of modified nucleotides (dUTP, dITP, biotin-, digoxigenin- and fluorescently-labeled nucleotides)
• Available in 2 different concentrations: 5 units/µL and 1 unit/µL
  

Taq DNA Polymerase is a highly thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity and a very low 5´→ 3´ exonuclease activity. The enzyme is supplied with a detergent-free, Mg-free Reaction Buffer, making it compatible with microarray and DHPLC applications and reactions that require adjustment of magnesium concentration.

Bio-Synthesis's Taq DNA Polymerase with 10X Taq Reaction Buffer (Mg-Free) is available in 2 convenient sizes:

1000 units at a concentration of 5000 units/mL (Cat # 28087)

Enzyme Source:
An E. coli strain with a cloned Taq DNA Polymerase gene from Thermus aquaticus YT-1

APPLICATIONS

• Routine PCR
• Preparation of PCR products for cloning
• Primer extension
• Microarray analysis
• DHPLC
• DNA labelling
• DNA sequencing

Figure 1. Successful Amplification of Different Sized Amplicons. Bio-Synthesis's Taq DNA Polymerase was used in a series of PCR reactions to amplify a number of different sized amplicons from the adenovirus E2 region. Lanes 1 and 2 correspond to a 189 bp product, Lanes 3 and 4 correspond to a 565 bp product, Lanes 5 and 6 correspond to a 1501 bp product, and Lanes 7 and 8 correspond to a 2202 bp product. Bio-Synthesis's Taq DNA Polymerase was able to amplify all the different sized products with a high sensitivity and yield. Lane M1 is Bio-Synthesis's PCR Sizer 100 bp DNA Ladder and Lane M2 is Bio-Synthesis's MidRanger 1kb DNA Ladder.

Reagents supplied - Cat# 28087:

1000 units of Taq DNA Polymerase (5000 units/mL)
4 x 1.25 mL of 10X Taq Reaction Buffer (Mg-Free)
1.25 mL of 25 mM MgCl2

Reaction Conditions:
1X Taq Reaction Buffer (Mg-Free) - (10 mM Tris-HCl, 50 mM KCl, PCR stabilizer, pH 8.3 @ 25°C)

Taq Storage Buffer:
50 mM Tris-HCl pH 8 at 25°C, 50 mM KCl, 50mM NaCl, 0.5 mM Dithiothreitol, 0.1 mM EDTA, 50% Glycerol, 1% Triton X-100

Storage Temperature:
-20°C
Long-Term Storage : Store in -20°C.
Product Usage
For laboratory research use only, not for diagnostic use! Not for use in humans.