FAF1 Antibody

FAF1 Antibody

Catalog# : 3993

Fas-associated protein 1 (FAF1) was initially identified as a Fas-binding pro-apoptotic protein that is component of the death-inducing signaling complex in Fas-mediated apoptosis (1). FAF1 can also induce apoptosis in the absence of extrinsic death signals when overexpressed although it does not contain typical death motifs such as the death domain, death effector domain, and caspase recruitment domain (1,2). Overexpression of FAF1 also decreases the basal level of NF-kappaB activity in transfected 293 cells, inhibits NF-kappaB activity induced by TNF-alpha, IL-1beta and lipopolysaccharide, and prevents NF-kappaB translocation to the nucleus (3), suggesting that another role of FAF1 is to negatively regulate the activity of NF-kappaB. FAF1 can also interact with the inflammatory signaling PYRIN-containing Apaf-1-like proteins (PYPAFs, also called NALPs) such as PYPAF1, PYPAF2 (NALP2), and PYPAF7, suggesting FAF1 may also be involved in the inflammation pathway (4). Multiple differentially spliced isoforms of FAF1 are known to exist.

Additional Names : FAF1 (CT), Fas-associated protein 1, hFAF1
Description
Left: Western blot analysis of FAF1 in Jurkat cell lysate with FAF1 antibody at (A) 1 and (B) 2 µg/ml.

Below: Immunohistochemistry of FAF1 in rat spleen tissue with FAF1 antibody at 2.5 µg/ml.

Other Product Images

Source : FAF1 antibody was raised against a 19 amino acid peptide from near the carboxy terminus of human FAF1.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : FAF1 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Application : FAF1 antibody can be used for detection of FAF1 by Western blot at 1 – 2 µg/ml.

Tested Application(s) : E, WB, IHC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat.No. 3993P - FAF1 Peptide

Long-Term Storage : FAF1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1. Cat. No. 1205 - Jurkat Cell Lysate
2. Cat. No. 1466 - Rat Spleen Tissue Lysate

Species Reactivity :H, M, R

GI Number : 5901948

Accession Number : NP_008982

Short Description : (CT) Fas-associated protein 1

References
1. Chu K, Niu X, and Williams LT. A Fas-associated protein factor, FAF1, potentiates Fas-mediated apoptosis. Proc. Natl. Acad. Sci. USA 1995; 92:11894-8.
2. Ryu SW and Kim E. Apoptosis induced by human Fas-associated factor 1, hFAF1, requires its ubiquitin homologous domain, but not the Fas-binding domain. Biochem. Biophys. Res. Commun. 2001; 286:1027-32.
3. Park M-Y, Jang HD, Lee SY, et al. Fas-associated Factor-1 inhibits Nuclear Factor-kappaB (NF-kappaB) activity by interfering with nuclear translocation of the RelA (p65) subunit of NF-kappaB. J. Biol. Chem. 2004; 279:2544-9.
4. Kinoshita T, Kondoh C, Hasegawa M, et al. Fas-associated factor 1 is a negative regulator of PYRIN-containing Apaf-1-like protein 1. Int. Immunol. 2006;18:1701-6.