Catalog# : 2147
Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as ASK-1 and RIP, are mediators of apoptosis. Two novel serine/threonine kinases that induce apoptosis were recently identified and designated DRAK1 and DRAK2 for DAP kinase-related apoptosis-inducing protein kinases (1). DRAKs contain an N-terminal kinase domain and a C-terminal regulation domain. Overexpression of DRAK1 induces apoptosis. DRAKs have high sequence homology to DAP and ZIP kinases, and they represent a novel family of serine/threonine kinases, which mediates apoptosis through their catalytic activities. DRAK1 is located in nucleus and the messenger RNA was ubiquitously expressed in human tissues (1).
Additional Names : DRAK1 (NT), DRAK1
DescriptionLeft: Western blot analysis of DRAK1 in (A) MOLT4 and (B) A431 whole cell lysates with DRAK1 antibody at 1 µg/ml.
Below: Immunocytochemistry staining of MOLT4 cells using DRAK1 antibody at 2 µg/ml.
Other Product Images
Source : DRAK1 antibody was raised against a peptide corresponding to amino acids near the amino terminus of human DRAK1.Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : DRAK1 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Human DRAK1 (N-Terminus) Peptide (Cat. No. 2147P)
Application : DRAK1 antibody can be used for detection of DRAK1 by Western blot at 0.5 – 1 µg/ml.A431 or MOLT4 whole cell lysate can be used as positive control and an approximately 50 kDa band can be detected. DRAK1 antibody is human specific, and has no cross responses to DRAK2, DAP or ZIP kinases.
Tested Application(s) : E, WB, ICC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 2147P - DRAK1 Peptide
Long-Term Storage : DRAK1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1.Cat. No. 1202 - A431 Cell Lysate
2.Cat. No. 1206 - MOLT4 Cell Lysate
Species Reactivity :H
GI Number : 14423930
Accession Number : Q9UEE5
Short Description : (NT) DAPK Related Apoptosis-inducing Kinase
References
1.Sanjo H, Kawai T, Akira S. DRAKs, novel serine/threonine kinases related to death-associated protein kinase that trigger apoptosis. J Biol Chem 1998;273:29066-71
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