DNase II Antibody

DNase II Antibody

Catalog# : 2059

Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins including AIF and Acinus . DNase II causes both chromatin condensation and DNA fragmentation . The genes encoding human (2-4), porcine (4), and murine (5) DNase II have been cloned. The DNase II gene encodes a 40 kDa proenzyme. The mature enzyme consists of two non-identical subunits, the 32 kDa (a) and 12 kDa (b) chains, generated by proteolytic processing. Overexpression of DNase II induces chromatin condensation (3). DNase II is ubiquitously expressed in human tissues.

Additional Names : DNase II
Description
Left: Western blot analysis of DNase II in human spleen tissue lysate in the absence (lane 1) or presence (lane 2) of blocking peptide with DNase antibody II at 1:500 dilution.

Below: Immunohistochemistry of DNaseII in human spleen tissue with DNaseII antibody at 5 µg/ml.

Other Product Images

Source : DNase II antibody was raised against a peptide corresponding to amino acids 347 to 360 of human DNase II precursor (2-4) .

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : DNase II antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Immunogen : Human DNase II (C-Terminus) Peptide (Cat. No. 2059P)

Application : DNase II antibody can be used for detection of DNase II expression by Western blot at 1:500 to 1:1000 dilution. An approximate 40 kDa band can be detected, which represents the pro-enzyme of DNase II.

Tested Application(s) : E, WB, IHC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 2059P - DNase II Peptide

Long-Term Storage : DNase II antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1.Cat. No. 1306 - Human Spleen Tissue Lysate
2.Cat. No. 1208 - THP-1 Whole Cell Lysate

Species Reactivity :H

GI Number : 2921838

Accession Number : AF047016

Short Description : DNase involved in apoptosis

References
1.Zamzami N, Kroemer G. Condensed matter in cell death. Nature 1999;401:127-8
2.Yasuda T, Takeshita H, Iida R, Nakajima T, Hosomi O, Nakashima Y, Kishi K. Molecular cloning of the cDNA encoding human deoxyribonuclease II. J Biol Chem 1998;273:2610-6
3.Krieser RJ, Eastman A. The cloning and expression of human deoxyribonuclease II. A possible role in apoptosis. J Biol Chem 1998;273:30909-14
4.Shiokawa D, Tanuma S. Cloning of cDNAs encoding porcine and human DNase II. Biochem Biophys Res Commun 1998;247:864-9