DR4 Antibody

DR4 Antibody

Catalog# : 1167

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors, TNFR1 and Fas. A novel death domain containing receptor was recently identified and designated DR4 (for death receptor 4)1. The ligand for this novel death receptor has been identified and termed TRAIL2,3, which is a new member in the TNF family. DR4 is also called TRAIL receptor-1 (TRAIL-R1)4. DR4 is expressed in most of human tissues including spleen, peripheral blood leukocytes, small intestine and thymus. Like TNFR1, Fas and DR3, DR4 mediates apoptosis and NF-kappaB activation in many tissues and cells.

Additional Names : DR4 (NT), TRAIL-R1
Description
Left: Western blot analysis of DR4 in HeLa (H), K562 (K), and Jurkat (J) whole cell lysate with DR4 antibody at 1:500 dilution.




Source : DR4 antibody was raised against a peptide corresponding to amino acid 1 to 20 of human DR4 mature protein.

Purification : Antibody is DEAE purified

Clonality and Clone : This is a polyclonal antibody.

Host : DR4 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Immunogen : Human DR4 (N-Terminus) Peptide (Cat. No. 1167P)

Application : DR4 antibody can be used for detection of DR4 by Western blot at 1:500 to 1:1000 dilution. A 57 kDa band can be detected. DR4 antibody has no cross reaction to DR5.

Tested Application(s) : E, WB

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 1167P - DR4 Peptide

Long-Term Storage : DR4 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1.Cat. No. 1201 - HeLa Cell Lysate
2.Cat. No. 1205 Jurkat Whole Cell Lysate

Species Reactivity :H

GI Number : 1945072

Accession Number : AAC51226

Short Description : (NT) Death Receptor for TRAIL

References
1.Pan G; O'Rourke K; Chinnaiyan AM; O'Rourke K; Gentz R; Ebner R; Ni J; Dixit VM. The receptor for the cytotoxic ligand TRAIL. Science; 1997;276:111-113
2.Wiley SR, Schooley K, Smolak PJ, Din WS, Huang CP, Nicholl JK, Sutherland GR, Smith TD, Rauch C, Smith CA, et al. Identification and characterization of a new member of the TNF family that induces apoptosis. Immunity 1995;3:673-682
3.Pitti RM; Marsters SA; Ruppert S; Donahue CJ; Moore A; Ashkenazi A. Induction of apoptosis by Apo-2 ligand, a new member of the tumor necrosis factor cytokine family. J. Biol. Chem. 1996;271:12687-90
4.Schneider P, Thome M, Burns K, Bodmer JL, Hofmann K, Kataoka T, Holler N, Tschopp J. TRAIL receptors 1 (DR4) and 2 (DR5) signal FADD-dependent apoptosis and activate NF-kappaB. Immunity 1997;7:831-836 (RD1299)