Alternate Name:
Catalog# : NB-28088
Price/Unit : $205.00
Unit : 1000 units (5 U/ul)
Recombinant source
- Functionality preserved at wide range of temperatures
- Highly reliable and reproducible reactions
- Supplied with detergent-free Reaction Buffer for compatibility with microarray and DHPL applications
- Compatible with the use of modified nucleotides (dUTP, dITP, biotin-, digoxigenin- and fluorescently-labeled nucleotides)
- Available in 2 different concentrations: 5 units/µL and 1 unit/µL
Taq DNA Polymerase is a highly thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity and a very low 5´→ 3´ exonuclease activity. The enzyme is supplied with detergent-free 10X Taq Reaction Buffer, making it compatible with microarray and DHPLC applications.
Bio-Synthesis's Taq DNA Polymerase with 10X Taq Reaction Buffer is available in 2 convenient sizes:
−1000 units at a concentration of 5000 units/mL (Cat # 28088)
Enzyme Source:
An E. coli strain with a cloned Taq DNA Polymerase gene from Thermus aquaticus YT-1
APPLICATIONS
- Routine PCR
- Preparation of PCR products for cloning
- Primer extension
- Microarray analysis
- DHPLC
- DNA labelling
- DNA sequencing
Reagents supplied - Cat# 28088:
1000 units of Taq DNA Polymerase (5000 units/mL)
4 x 1.25 mL Taq Reaction Buffer (10X)
Reaction Conditions:
1X Taq Reaction Buffer (10 mM Tris-HCl, 50 mM KCl, 1.5 mM MgCl2, PCR stabilizer, pH 8.3 @ 25°C)
Storage Buffer:
- 50 mM Tris-HCl pH 8 at 25°C, 50 mM KCl, 50mM NaCl, 0.5 mM Dithiothreitol, 0.1 mM EDTA, 50% Glycerol, 1% Triton X-100
- Long-Term Storage : -20°C
- Product Usage
- For laboratory research use only, not for diagnostic use! Not for use in humans.
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