DEDD2 Antibody

DEDD2 Antibody

Catalog# : 3071

Apoptotic signals are often triggered by cell surface death receptors through protein-protein interactions mediated by conserved domains such as the death effector domain (1). A novel death effector domain (DED)-containing protein, DEDD2, has been recently identified and its over-expression in transfected cells induces moderate apoptosis and results in substantial sensitization to apoptosis induced by Fas, TRAIL, and FADD (2). More recently, work has shown that DEDD2 can bind caspase-8 and -10 in addition to FLIP but not FADD (2,3). Like the related protein DEDD, DEDD2 translocates from the cytosol to the nucleus upon induction of apoptosis, and it has been suggested that DEDD2 may target caspase-8 to the nucleus and that DEDD2 thus plays a critical role in death receptor-induced apoptosis (3). At least two alternatively spliced transcript variants encoding distinct isoforms have been found for DEDD2.

Additional Names : DEDD2, DNA-binding death effector domain-containing protein 2, FLAME-3
Description
Left: Western blot analysis of DEDD2 in RAW264.7 cell lysate with DEDD2 antibody at (A) 0.5, (B) 1 and (C) 2 μg/ml.



Source : DEDD2 antibody was raised against a peptide corresponding to 11 amino acids near the amino-terminus of human DEDD2.

Purification : Immunoaffinity chromatography purified IgG

Clonality and Clone : This is a polyclonal antibody.

Host : DEDD2 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Immunogen : Human DEDD2 Peptide (Cat. No. 3071P)

Application : DEDD2 antibody can be used for detection of DEDD2 by Western blot at 0.5 to 1 µg/ml.

Tested Application(s) : E, WB

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 3071P - DEDD2 Peptide

Long-Term Storage : DEDD2 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1.Cat. No. 1283 - RAW264.7 Cell Lysate

Species Reactivity :H, M, R

GI Number : 19923050

Accession Number : NP_579874

Short Description : A novel death effector domain containing protein

References
1.Tibbetts MD, Zheng L, and Lenardo MJ. The death effector domain protein family: regulators of cellular homeostasis. Nat. Immunol. 2003; 4:404-9.
2.Roth W, Stenner-Liewen F, Pawlowski K, et al. Identification and characterization of DEDD2, a death effector domain-containing protein. J. Biol. Chem. 2002; 277:7501-8.
3.Alcivar A, Hu S, Tang J, et al. DEDD and DEDD2 associate with caspase-8/10 and signal cell death. Oncogene 2003; 22:291-7.