F1A alpha Antibody

F1A alpha Antibody

Catalog# : 2279

Fas and tumor necrosis factor receptor 1 (TNFR1) are two prototype members in the death receptor family. A novel protein that associates with the intracellular domains of Fas and TNFR1 was recently identified and designated F1Aalpha and FEM1b (1,2). F1Aalpha/FEM1beta is the homologue of C. elegans sex determining protein FEM-1 (1,2). FEM-1/F1Aalpha is cleaved by CED-3 and caspase (3). FEM-1/F1Aalpha associates with CED-4 and its mammalian homologue Apaf-1 (3). Overexpression of F1Aalpha induces apoptosis. F1Aalpha is therefore a novel member of the death receptor associated protein that mediates apoptosis. F1Aalpha is expressed in a variety of human and mouse tissues (1,2)

Additional Names : F1A alpha (CT), F1A alpha
Description
Left: Western blot analysis of F1A alpha in mouse (A) and rat (B) liver tissue lysates with F1A alpha antibody at 1 µg/ml.

Below: Immunohistochemistry of F1Aalpha in mouse liver tissue with F1Aalpha antibody at 5 µg/ml.

Other Product Images

Source : F1A alpha antibody was raised against a peptide corresponding to amino acids 609 to 622 of human F1A alpha. This sequence is identical to the corresponding sequence of mouse FEM1b .

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : F1A alpha antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Immunogen : Human F1A alpha Peptide (Cat. No. 2279P)

Application : F1Ab antibody can be used for detection of F1Ab by Western blot at 0.5 to 1 µg/ml.An approximately 70 kDa band can be detected. It is human, mouse, and rat reactive.

Tested Application(s) : E, WB, IHC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 2279P - F1A alpha Peptide

Long-Term Storage : F1A alpha antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1. Cat. No. 1404 - Mouse Liver Tissue Lysate
2. Cat. No. 1464 - Rat Liver Tissue Lysate

Species Reactivity :H, M, R

GI Number : 6175869

Accession Number : AAF05314

Short Description : (CT) A novel death receptor-binding protein

References
1. Chan SL, Tan KO, Zhang L, Yee KS , Ronca F, Chan MY, Yu VC. F1Aalpha, a death receptor-binding protein homologous to the Caenorhabditis elegans sex-determining protein, FEM-1, is a caspase substrate that mediates apoptosis. J Biol Chem. 1999;274(45):32461-8.
2. Ventura-Holman T, Maher JF. Sequence, organization, and expression of the human FEM1B gene. Biochem Biophys Res Commun 2000;267(1):317-20
3. Chan SL, Yee KS , Tan KM, Yu VC. The Caenorhabditis elegans sex determination protein FEM-1 is a CED-3 substrate that associates with CED-4 and mediates apoptosis in mammalian cells. J Biol Chem. 2000;275(24):17925-8. (WD0102)