FAIM Antibody

FAIM Antibody

Catalog# : 2309

The susceptibility of primary splenic B cells to Fas-mediated apoptosis is regulated in a receptor-specific fashion. Terminal effectors of B cell Fas-resistance include the known anti-apoptotic proteins Bcl-xL, FLIP, and a recently identified protein termed FAIM. This molecule is broadly expressed in various tissues and exists in at least three isoforms. It is thought that resistance to Fas killing via increased expression of FAIM protects foreign antigen-specific B cells during interactions with FasL-bearing T cells whereas autoreactive B cells are deleted via Fas-dependent cytotoxicity. More recent results have indicated that FAIM interacts with both Trk and p75 neurotrophin receptor and may play a role in promoting neurite outgrowth in different neuronal systems by a mechanism involving the activation of NF-kappaB and the Ras-ERK pathway.

Additional Names : FAIM, Fas apoptotic inhibitory molecule
Description
Left: Western blot analysis of FAIM in human spleen tissue lysate with FAIM antibody at (A) 5 and (B) 10 µg/ml.





Source : FAIM antibody was raised against a 14 amino acid peptide from near the carboxy terminus of human FAIM .

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : FAIM antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Application : FAIM antibody can be used for detection of FAIM by Western blot at 5 – 10 µg/ml.

Tested Application(s) : E, WB

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat.No. 2309P - FAIM Peptide

Long-Term Storage : FAIM antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1. Cat. No. 1306 - Human Spleen Tissue Lysate

Species Reactivity :H, M

GI Number : 8922536

Accession Number : NP_060617

Short Description : an apoptosis inhibitor

References
1. Rothstein TL. Inducible resistance to Fas-mediated apoptosis in B cells. Cell Res. 2000; 10:245-66.
2. Schneider TJ, Fischer GM, Donohoe TJ, et al. A novel gene coding for a Fas apoptosis inhibitory molecule (FAIM) isolated from inducibly Fas-resistant B lymphocytes. J. Exp. Med. 1999; 189:949-55.
3. Sole C, Dolcet X, Segura MF, et al. The death receptor antagonist FAIM promotes neurite outgrowth by a mechanism that depends on ERK and NF-kappa B signaling. J. Cell Biol. 2004; 167:479-92.