Catalog# : NB-23800
Price/Unit : $240.00
Unit : 20 preps
For the rapid and efficient purification, cleanup and concentration of RNA or DNA
* Purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA)
* RNA is preferentially purified from other reaction components such as proteins and nucleotides, without the use of phenol or chloroform
* Purifies all sizes of DNA, from small PCR products to plasmids to genomic DNA.
* Rapid and efficient spin-column format
Features and Benefits | Specifications
BSI's CleanAll RNA/DNA Clean-Up and Concentration Micro Kit provides a rapid method for the purification, cleanup and concentration of RNA or DNA from different isolation methods or upstream applications. The kit can be used as an alternative to organic extraction and ethanol precipitation to clean up various enzymatic reactions. The CleanAll Kit purifies RNA from phenol/guanidine-based protocols or from various upstream enzymatic reactions such as DNase treatment, labeling and in vitro transcription. Furthermore, endotoxins can be removed from previously purified RNA solutions. The kit can be used to clean up DNA from digestions, ligations, PCR reactions, labeling reactions, DNA modification reactions and staining. The kit also provides a protocol for the rapid removal of endotoxins from previously purified DNA down to 0.1 EU/µg DNA or less.
Purification is based on spin column chromatography using BSI’s proprietary resin as the separation matrix. Briefly, the samples or reaction containing RNA or DNA is mixed with Binding Solution and the nucleic acids are bound to BSI's column (BIND). Under these conditions only the nucleic acids will bind to BSI's resin while most of the contaminating proteins and nucleotides are removed in the flowthrough. The bound nucleic acid is then washed to remove any remaining impurities (WASH). Lastly, the purified total RNA or DNA is eluted into 50 µL of the provided Elution Buffer or water (ELUTE). Please see procedure flowchart to the right.
FEATURES AND BENEFITS
* Versatile performance - Both DNA and RNA can be cleaned and concentrated using the same kit. Clean-up nucleic acids from a variety of isolation methods and upstream enzymatic reactions.
* Fast and easy processing - Rapid spin-column format allows for the processing of 10 samples in 20 minutes.
* Suitable for all sizes of RNA - Purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA).
* Suitable for all sizes of DNA - Purify all sizes of DNA, from small PCR products, native or linearized plasmids, to genomic DNA. Using an alternative protocol, even oligonucleotides and smaller DNA fragments can be purified.
* Optional endotoxin removal - Optional protocol is provided for the rapid removal of endotoxins from previously purified RNA or DNA.
* No phenol:chlorofom extractions - BSI’s CleanAll kit purifies RNA without the use of harmful chemicals such as phenol or chloroform
APPLICATIONS AND DATA
The kit can be used to clean up RNA and DNA from upstream applications such as:
* Digestions
* Ligations
* PCR reactions
* Labeling reactions
* DNA modification reactions
* Staining
Figure 1. High Recoveries and Efficient Endotoxin Removal. High recoveries of DNA can be seen when using the CleanAll kit, and these high recoveries are not compromised even during endotoxin removal. In Panel A, 1 µg of a PCR product was cleaned using the CleanAll RNA/DNA Clean-Up and Concentration Kit as well as BSI's PCR Purification Kit. More than 0.9 µg of the DNA was recovered from both kits with a comparable percent recovery of over 90%. In Panel B, 10 µg of plasmid DNA preparation was cleaned from endotoxin by using the CleanAll RNA/DNA Kit. Endotoxin levels of the plasmid DNA sample were reduced from 0.75 EU/µg plasmid DNA to less 0.05 EU/µg plasmid DNA. Approximately 95% of the endotoxin units in the DNA sample were removed using the kit, therefore the kit allows for high recoveries coupled with exceptional cleanup.
Figure 2: Clean-Up of RNA with High Recovery. BSI’s CleanAll RNA/DNA Clean-Up and Concentration Kit can be used to clean up various enzymatic reactions including DNase treatment. In Figure 2 above, Lane 1 is the RNA input, while lanes 2-5 contain the RNA that has been cleaned using BSI’s CleanAll Kit. It can be seen that in all cases the recovery is high, and the purified RNA is intact and of a high quality.
CleanAll RNA/DNA Clean-Up and Concentration Kit Components
1. Binding Solution
2. Wash Solution
3. Elution Buffer
4. Micro Spin Columns
5. Collection Tubes
6. Elution tubes
7. Product Insert
Long-Term Storage : All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
Product Usage
For laboratory research use only, not for diagnostic use! Not for use in humans.
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