EndoG Monoclonal Antibody

EndoG Monoclonal Antibody

Catalog# : PM-4581

The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (EndoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD. EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans. EndoG also cleaves DNA in vitro.

Additional Names : EndoG (7F2D7): Endonuclease G
Source : Mouse monoclonal EndoG antibody was raised against a recombinant protein corresponding to amino acids 51 – 140 of human EndoG.






Source : Mouse monoclonal EndoG antibody was raised against a recombinant protein corresponding to amino acids 51 – 140 of human EndoG.

Purification : Immunoaffinity chromotography purified IgG

Clonality and Clone : This is a monoclonal antibody. (Clone 7F2D7)

Host : EndoG monoclonal antibody was raised in mouse. Please use anti-rabbit secondary antibodies.

Immunogen : Recombinant protein corresponding to amino acids 51 – 140 of human EndoG.

Application : EndoG monoclonal antibody can be used for detection of EndoG by Western blot at 5 – 10 µg/ml.

Tested Application(s) : E, WB

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Long-Term Storage : EndoG monoclonal antibody can be stored at 4ºC, stable for one year.

Positive Control
1.Cat. No. 1211 - HepG2 Cell Lysate
2.Cat. No. 95-103 - EndoG Recombinant Protein

Species Reactivity :H, M, R

Short Description : Endonclease G

References
1.Li LY, Luo X, Wang X. Endonuclease G is an apoptotic DNase when released from mitochondria. Nature 2001; 412:95-9.
2.Parrish J, Li L, Klotz K, et al. Mitochondrial endonuclease G is important for apoptosis in C. elegans. Nature 2001; 412:90-4
3.Hengartner MO. Apoptosis. DNA destroyers. Nature 2001; 412:27, 29.
4.Widlak P, Li LY, Wang X, et al. Action of recombinant human apoptotic endonuclease G on naked DNA and chromatin substrates: cooperation with exonuclease and DNase I. J. Biol. Chem. 2001; 276:48404-9.