Inclusion Body Solubilization Reagent

Inclusion Body Solubilization Reagent

Catalog# : NB-18700
Price/Unit : $71.80
Unit : 25 mL

For the efficient solubilization of inclusion body proteins

* Fast dissolving of inclusion body aggregates
* Allows for downstream applications of inclusion body proteins

Bio-Synthesis's Inclusion Body Solubilization Reagent dissolves inclusion body aggregates resulting from the expression of recombinant proteins in bacteria. This reagent has demonstrated exceptional ablility to solubilize inclusion bodies. Once dissolved, proteins can be analyzed by SDS-PAGE, quantified or further purified for refolding.

Inclusion bodies can be released from bacterial cells using Bio-Synthesis's Cell Lysis Reagent (available separately). Bio-Synthesis's Inclusion Body Protein Isolation Kits can also be used as complete kits for cell disruption, inclusion body solubilisation and purification using spin column chromatography

APPLICATIONS

Solubilized proteins can be used in a number of downstream applications including:

* SDS-PAGE analysis
* Western blot
* ELISA
* Refolding experiments
* Mass spectrometry
* Further purification and scale up

Figure 1: Efficient Isolation of Inclusion Body Proteins Using Bio-Synthesis's Cell Lysis Reagent and Inclusion Body Solubilization Reagent. Inclusion Bodies were extracted and solubilized following gene expression. Recovered proteins were analyzed on 12% SDS-PAGE and stained with Coomassie Brilliant Blue R-250. Numbers represent kDa sizes of protein bands.
Product Usage
For laboratory research use only, not for diagnostic use! Not for use in humans.