ProteoSpin Total Protein Concentration Kit

ProteoSpin Total Protein Concentration Kit

Catalog# : NB-22000
Purity : Purity > 95% by HPLC
Solubility : Distilled water for a solution up to 2 mg/ml, otherwise we recommend using DMSO
Format : Each vial contains lyophilized solid packaged and supplied as a trifluoroacetate salt
Price/Unit : $230.00
Unit : 25 preps

For the rapid concentration and desalting of proteins

* Simultaneous concentration and desalting of proteins.
* No molecular weight cutoff.
* Rapid and convenient spin-column format.

The ProteoSpinTM Total Protein Concentration Micro Kit provides a fast and a simple procedure for the concentration of protein samples many fold in a convenient spin column format. The kit employs an innovative separation material that is able to concentrate proteins and peptides while simultaneoulsy removing salts and other contaminants such as detergents and denaturants. One major advantage to this kit is there is no molecular weight cut-off, unlike other products from other vendors. Therefore a broad size range of proteins from peptides to antibodies can be processed. Another advantage of this kit is that it concentrates, removes salts and offers buffer exchange at the same time, whereas other products do these processes as separate procedures and therefore saves considerable time and expense.

The kit employs a proprietary matrix which acts as an ion exchanger and is able to concentrate proteins many fold as well as remove salts. Breifly, pH Binding Buffer is added to the protein sample and the sample is applied to Bio-Synthesis' column (BIND). Under these conditions the proteins will bind to the column while salts and other contaminants are removed in the flowthrough. The bound proteins are then washed to remove any remaining impurities (WASH). Lastly, the clean proteins are eluted into a small volume of the provided Elution Buffer or into other optional elution buffers (user-provided) (ELUTE). Please see the procedure flowchart to the right.

Each spin column can process up to 200 µg of protein. The kit can efficiently process 12 samples in only 20 minutes using an easy-to use protocol. The percentage of protein recovery is up to 99%, and the percentage of salt removal is up to 99%. The concentrated proteins are ready for any downstream proteomic applications including 2D SDS-PAGE, Whole protein mass spectrometry, Isoelectric focusing, X-ray crystallography, NMR spectroscopy and Protein microarrays

FEATURES AND BENEFITS

* Fast and easy processing - Efficiently process 12 samples in only 20 minutes using an easy-to-use protocol.
* High yield - Recovery of up to 99% of input amount.
* No mixing or formulation - Solutions and protocols are provided with no need for formulation.
* No molecular weight cutoff - Based on an ion exchange mechanism, a broad size range of proteins (from peptides to large proteins) can be processed.
* Proteins bind while salts are discarded in flowthrough - Bio-Synthesis' resin has an inherent low affinity for salt but high affinity for proteins, providing salt removal and buffer exchange simultaneously.
* Suitable for downstream applications - Final elution performed with volatile or non-volatile buffers suitable for: Mass spectrometry, SDS-PAGE, Isoelectric focusing, X-ray crystallography, NMR spectroscopy.

APPLICATIONS

Final elution performed with volatile or non-volatile buffers suitable for:

* Mass spectrometry
* SDS-PAGE
* Isoelectric focusing
* X-ray crystallography
* NMR spectroscopy
Total Protein Concentration Kit Contents
1 Column Activation and Wash Buffer
2 pH Binding Buffer
3 Elution Buffer
4 Neutralizer
5 Micro Spin Columns (25)
6 Collection Tubes (25)
7 Elution Tubes (25)
8 Product Insert

Storage Conditions

Unopened solutions should be stored at room temperature. Once opened, the solutions should be stored at 4°C when not in use.
Long-Term Storage : Store the peptide at -20°C. Keep container tightly
Product Usage
For laboratory research use only, not for diagnostic use! Not for use in humans.