PD-1 Antibody

PD-1 Antibody

Catalog# : 4067

Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of “danger signals” such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.

Additional Names : PD-1 (IN), Programmed cell death 1, PDCD1, CD279
Description
Left: Western blot analysis of PD-1 in THP-1 cell lysate with PD-1 antibody at 1 µg/ml in the (A) absence and (B) presence of blocking peptide.

Below: Immunohistochemistry of PD-1 in human brain tissue with PD-1 antibody at 5 µg/ml.

Other Product Images

Source : PD-1 antibody was raised against a 16 amino acid peptide from near the center of human PD-1.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : PD-1 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Application : PD-1 antibody can be used for detection of PD-1 by Western blot at 0.5 – 1 µg/ml.

Tested Application(s) : E, WB, IHC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat.No. 4067P - PD-1 Peptide

Long-Term Storage : PD-1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1. Cat. No. 1208 - THP-1 Cell Lysate

Species Reactivity :H, M, R

GI Number : 145559515

Accession Number : Q15116

Short Description : (IN) Programmed cell death 1 protein

References

1. Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90.
2. Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95.
3. Zhong X, Bai C, Gao W, et al. Suppression of expression and function of negative immune regulator PD-1 by certain pattern recognition and cytokine receptor signals associated with immune system danger. Int. Immunol. 2004; 16:1181-8.
4. Nishimura H, Nose M, Hiai H, et al. Development of lupus-like autoimmune diseases by the disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor. Immunity 1999; 11:141-51.