Catalog# : 2143
Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNF-R1 and Fas. Several novel death receptors including DR3, DR4, DR5, and DR6 were recently identified. Cell death signal is transduced by death domain containing adapter molecules through the interaction with death domain of these death receptors. A novel TNF-R1 interacting protein was recently identified and designated SODD for silencer of death domains (1). SODD associates with the death domain of TNF-R1 and prevents constitutive activation of TNF-R1 signaling. TNF treatment releases SODD and permits adapter molecules such as TRADD recruiting to the active TNF-R1 complex, which activates TNF signaling pathways. SODD also interacts with DR3. SODD is ubiquitously expressed in human tissues and cell lines.
Additional Names : SODD (NT), SODD
Left: Western blot analysis of SODD in HeLa (1,3) and THP-1 (2,4) whole cell lysates in the absence (1,2) or presence (3,4) of blocking peptide (Catalog no. 2143P) with SODD antibody at 1:500 dilution.
Below: Immunocytochemistry of SODD in HeLa cells with SODD antibody at 5 µg/ml.
Other Product Images
Source : SODD antibody was raised against a peptide corresponding to amino acids near the amino terminus of human SODD.
Purification : Antibody is DEAE purified
Clonality and Clone : This is a polyclonal antibody.
Host : SODD antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Human SODD (N-Terminus) Peptide (Cat. No. 2143P)
Application : SODD antibody can be used for detection of SODD by Western blot at 1:500 to 1:2000 dilution. An approximately 60 kDa band can be detected.
Tested Application(s) : E, WB, ICC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 2143P - SODD Peptide
Long-Term Storage : SODD antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1. Cat. No. 1201 - HeLa Cell Lysate
2. Cat. No. 1208 - THP-1 Whole Cell Lysate
Species Reactivity :H, M, R
GI Number : 4160013
Accession Number : AF111116
Short Description : (NT) Silencer Of Death Domains
1. Jiang Y, Woronicz JD, Liu W, Goeddel DY. Prevention of constitutive TNF receptor 1 signaling by silencer of death domains. Science 1999;283:543-6 (RD1299)