Tuesday, August 31, 2010

Rkhd4 Antibody

Rkhd4 Antibody

Catalog# : 4869

Rkhd4, also known as MEX3A is a member of a novel family of four homologous human MEX3 proteins each containing two heterogeneous nuclear ribonucleoprotein K homology (KH) domains and one carboxy-terminal RING finger module. MEX3 proteins, including Rkhd4, are phosphoproteins that bind RNA through their KH domains and shuttle between the nucleus and the cytoplasm via the CRM1 export pathway. These proteins are a novel family of evolutionarily conserved RNA-binding proteins, differentially recruited to P bodies and potentially involved in post-transcriptional regulatory mechanisms. While Rkhd2 has been suggested to be associated with susceptibility to essential hypertension type 8, the function of Rkhd4 remains unknown. Rkhd3 and Rkhd4, but not Rkhd2, co-localize with both the hDcp1a decapping factor and Argonaute (Ago) proteins in processing bodies (P bodies), recently characterized as centers of mRNA turnover.

Additional Names : Rkhd4, MEX3A, MEX3-A

Left: Western blot analysis of Rkhd4 in SK-N-SH cell lysate with Rkhd4 antibody at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide.

Below: Immunohistochemistry of Rkhd4 in human brain tissue with Rkhd4 antibody at 2.5 µg/ml.

Other Product Images
Source : Rkhd4 antibody was raised against a 14 amino acid peptide from near the carboxy terminus of human Rkhd4.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : Rkhd4 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Application : Rkhd4 antibody can be used for detection of Rkhd4 by Western blot at 1 - 2 µg/ml.

Tested Application(s) : E, WB, IHC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat.No. 4869P - Rkhd4 Peptide

Long-Term Storage : Rkhd4 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1. Cat. No. 1220 - SK-N-SH Cell Lysate

Species Reactivity :H, M, R

GI Number : 134034168

Accession Number : A1L020

Short Description : RNA binding, Bcl-2, apoptosis, Mex3, decapping

1. Draper BW, Mello CC, Bowerman B, et al. MEX-3 is a KH domain protein that regulates blastomere identity in early C. elegans embryos. Cell 1996; 87:205–16.
2. Liu J, Valencia-Sanchez MA, Hannon GJ, et al. MicroRNA-dependent localization of targeted mRNAs to mammalian P-bodies. Nat. Cell Biol 2005; 7:719–23.
3. Guzman B, Cormand B, Ribases M, et al. Implication of chromosome 18 in hypertension by sibling pair and association analyses: putative involvement of the RKHD2 gene. Hypertension 2006; 48:883-91.
4. Buchet-Poyau K, Courchet J, Le Hir H, et al. Identification and characterization of human Mex-3 proteins, a novel family of evolutionarily conserved RNA-binding proteins differentially localized to processing bodies. Nucleic Acids Res. 2007; 35:1289-300.

No comments: