Monday, August 30, 2010

Rkhd2 Antibody

Rkhd2 Antibody

Catalog# : 4857

Rkhd2, also known as MEX3C is a member of a novel family of four homologous human MEX3 proteins each containing two heterogeneous nuclear ribonucleoprotein K homology (KH) domains and one carboxy-terminal RING finger module. MEX3 proteins, including Rkhd2, are phosphoproteins that bind RNA through their KH domains and shuttle between the nucleus and the cytoplasm via the CRM1 export pathway. These proteins are a novel family of evolutionarily conserved RNA-binding proteins, differentially recruited to P bodies and potentially involved in post-transcriptional regulatory mechanisms. It has been suggested that genetic variations in Rkhd2 may be associated with susceptibility to essential hypertension type 8. Rkhd3 and Rkhd4, but not Rkhd2, co-localize with both the hDcp1a decapping factor and Argonaute (Ago) proteins in processing bodies (P bodies), recently characterized as centers of mRNA turnover.

Additional Names : Rkhd2, RING finger and KH domain-containing protein 2, RING finger protein 194, MEX3C, RNF194

Left: Western blot analysis of Rkhd2 in rat heart tissue lysate with Rkhd2 antibody at (A) 0.5 µg/ml and (B) 1 µg/ml.

Source : Rkhd2 antibody was raised against a 15 amino acid peptide from near the carboxy terminus of human RKHD2.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : Rkhd2 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Application : Rkhd2 antibody can be used for detection of Rkhd2 by Western blot at 0.5 – 1 µg/ml.

Tested Application(s) : E, WB

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat.No. 4857P - Rkhd2 Peptide

Long-Term Storage : Rkhd2 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1. Cat. No. 1461 - Rat Heart Tissue Lysate

Species Reactivity :H, M, R

GI Number : 148229134

Accession Number : NP_057710

Short Description : RING finger and KH domain-containing protein 2

1. Draper BW, Mello CC, Bowerman B, et al. MEX-3 is a KH domain protein that regulates blastomere identity in early C. elegans embryos. Cell 1996; 87:205–16.
2. Liu J, Valencia-Sanchez MA, Hannon GJ, et al. MicroRNA-dependent localization of targeted mRNAs to mammalian P-bodies. Nat. Cell Biol 2005; 7:719–23.
3. Guzman B, Cormand B, Ribases M, et al. Implication of chromosome 18 in hypertension by sibling pair and association analyses: putative involvement of the RKHD2 gene. Hypertension 2006; 48:883-91.
4. Buchet-Poyau K, Courchet J, Le Hir H, et al. Identification and characterization of human Mex-3 proteins, a novel family of evolutionarily conserved RNA-binding proteins differentially localized to processing bodies. Nucleic Acids Res. 2007; 35:1289-300.

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