Tuesday, August 31, 2010

Smac Antibody

Smac Antibody

Catalog# : 2409

The inhibitor of apoptosis proteins (IAPs) regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes the inhibitory effect of IAPs on caspases was recently identified and designated Smac/DIABLO (1,2). Smac/DIABLO is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates cytochrome c/Apaf-1/capase-9 pathway. Analysis of the structural basis of Smac/DIABLO reveals that the N-terminal amino acids are required for binding of Smac/DIABLO to IAPs and activation of caspases (3-6). Smac/DIABLO is expressed in a variety of human and mouse tissues (1,2).

Additional Names : Smac (CT), DIABLO
Left: Western blot analysis of Smac in human heart tissue lysate in the absence (A) or presence (B) of blocking peptide (2409P) with Smac antibody at 1 µg /ml.

Below: Immunohistochemistry of Smac/DIABLO in human ovary tissue with Smac antibody at 5 µg/ml.

Other Product Images

Source : Smac antibody was raised against a peptide corresponding to amino acids 225 to 239 of human Smac/DIABLO .

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : Smac antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Immunogen : Human Smac / DIABLO (C-Terminus) Peptide (Cat. No. 2409P)

Application : Smac antibody can be used for detection of Smac/DIABLO by Western blot at 0.5 to 1 µg/ml.Human heart tissue lysate can be used as positive control and a 25 kDa band can be detected.

Tested Application(s) : E, WB, IHC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 2409P - Smac Peptide

Long-Term Storage : Smac antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1. Cat. No. 1301 - Human Heart Tissue Lysate
2. Cat. No. 1316 - Human Ovary Tissue Lysate

Species Reactivity :H, M, R

GI Number : 9454219

Accession Number : AAF87716

Short Description : (CT) IAPs binding protein

1. Du C, Fang M, Li Y, Li L, Wang X. Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition. M Cell. 2000;102(1):33-42.
2. Verhagen AM, Ekert PG, Pakusch M, Silke J, Connolly LM, Reid GE, Moritz RL, Simpson RJ, Vaux DL. Identification of DIABLO, a mammalian protein that promotes apoptosis by binding to and antagonizing IAP proteins. Cell. 2000;102(1):43-53.
3. Srinivasula SM, Datta P, Fan XJ, Fernandes-Alnemri T, Huang Z, Alnemri ES. Molecular Determinants of the Caspase-promoting Activity of Smac/DIABLO and Its Role in the Death Receptor Pathway. J Biol Chem. 2000;275(46):36152-36157.
4. Chai J, Du C, Wu JW, Kyin S, Wang X, Shi Y. Structural and biochemical basis of apoptotic activation by Smac/DIABLO. Nature. 2000;406(6798):855-62.

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