FLASH Antibody

FLASH Antibody

Catalog# : 2271

A novel mammalian CED-4 homologous was recently identified and cloned in mouse (1) and human (2) and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8 (1). There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity (1,2). FLASH is widely expressed.

Additional Names : FLASH (CT), FLASH

Description
Left: Western blot analysis of FLASH in HeLa whole cell lysate with FLASH antibody at 0.5 µg/ml.

Below: Immunocytochemistry of FLASH in HeLa cells with FLASH antibody at 10 µg/ml.

Other Product Images

Source : FLASH antibody was raised against a synthetic peptide corresponding to amino acids near the carboxy-terminus of human FLASH which differ from those of mouse by one amino acid.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : FLASH antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Immunogen : Human FLASH (C-Terminus) Peptide (Cat. No. 2271P)

Application : FLASH antibody can be used for detection of FLASH by Western blot at 0.25 to 0.5 µg/ml.An approximate 220 kDa band can be detected.

Tested Application(s) : E, WB, ICC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 2271P - FLASH Peptide

Long-Term Storage : FLASH antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1.Cat. No. 1201 - HeLa Cell Lysate

Species Reactivity :H

GI Number : 5533376

Accession Number : AF165161

Short Description : (CT) Homolog of CED4

References
1.Imai Y, Kimura T, Murakami A, Yajima N, Sakamaki K, Yonehara S. The CED-4-homologous protein FLASH is involved in Fas-mediated activation of caspase-8 during apoptosis. Nature 1999;398:777-85
2.Koonin EV, Aravind L, Hofmann K, Tschopp J, Dixit VM Apoptosis. Searching for FLASH domains. Nature 1999;401:662-3
3.Medema JP. Apoptosis. Life and death in a FLASH. Nature 1999;398:756-7 (WD0800)