The sorting of acid hydrolases to lysosomes rely on mannose 6-phosphate receptors that cycle between the trans-Golgi network (TGN) and endosomes. The maintenance of this cycle requires the function of the mammalian Golgi-associated retrograde protein (GARP) complex which is composed of three subunits: VPS52, VPS53, and VPS54. Depletion of any of these three proteins, such as by RNAi, impairs the retrograde transport of multiple TGN proteins. VPS53 was identified as an HIV dependency factor (HDF) and plays a role in viral entry to the cell, suggesting that VPS53 may be an important drug target in HIV treatment. At least five isoforms of VPS53 are known to exist.
Additional Names : VPS53 (CT), Vacuolar protein sorting 53, HCCS1
Left: Western blot analysis of VPS53 in 293 cell lysate with VPS53 antibody at (A) 0.5 and (B) 1 µg/ml.
BelowImmunocytochemistry of VPS53 in 293 cells with VPS53 antibody at 5 μg/ml.
Other Product Images
Source :VPS53 antibody was raised against a 18 amino acid peptide from near the carboxy terminus of human VPS53.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : VPS53 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application : VPS53 antibody can be used for detection of VPS53 by Western blot at 0.5 – 1 µg/ml.
Tested Application(s) : E, WB, ICC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4593P - VPS53 Peptide
Long-Term Storage : VPS53 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1210 - 293 Cell Lysate
Species Reactivity : H, M, R
GI Number : 119611064
Accession Number : EAW90658
Short Description : (CT) A vacuolar sorting protein
1. Pfeffer SR. Targeting of proteins to the lysosome. Curr. Top. Microbiol. Immunol. 1991; 170:43-65.
2. Liewen H, Meinhold-Heerlein I, Oliveira V, et al. Characterization of the human GARP (Golgi associated retrograde protein) complex. Exp. Cell Res. 2005; 306:22-34.
3. Perez-Victoria FJ, Mardones GA, and Bonifacino JS. Requirement of the human GARP complex for mannose 6-phosphate-receptor-dependent sorting of cathepsin D to lysosomes. Mol. Biol. Cell 2008; 19:2350-62.
4. Brass AL, Dykxhoorn DM, Benita Y, et al. Identification of host proteins required for HIV infection through a functional genomic screen. Science 2008; 319:921-6.