ZCRB1 is a nuclear protein first identified in a differential display screen involving morphine-dependence-related genes. Its expression is increased following morphine treatment and has been observed to be elevated in HepG2 cells. ZCRB1 contains a CCHC-type zing finger RNA-binding motif and can interact with the DBA-binding domain of the stem cell regulator C/EBP transcription factors. ZCRB1 was identified as one of the protein components of U11/U12 snRNPs, which are components of U12-type spliceosome and function as a molecular bridge connecting both ends of the intron, suggesting ZCRB1 may play a key role in U12-type splicing.
Additional Names : ZCRB1, Zinc finger CCHC-type and RNA binding motif 1, ZCCHC19, MADP-1, RBM36
Left: Western blot analysis of ZCRB1 in Raji cell lysate with ZCRB1 antibody at (A) 1 and (B) 2 µg/ml.
Source :ZCRB1 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human ZCRB1.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : ZCRB1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application : ZCRB1 antibody can be used for detection of ZCRB1 by Western blot at 1 - 2 µg/ml.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 5225P - ZCRB1 Peptide
Long-Term Storage : ZCRB1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1207 - Raji Cell Lysate
Species Reactivity : H, M, R
GI Number : 51243065
Accession Number : NP_149105
Short Description : Zinc finger CCHC-type and RNA binding motif 1
1. Wang H, Gao MX, Li L, et al. Isolation, expression, and characterization of the human ZCRB1 gene mapped to 12q2. Genomics 2007; 89:59-69.
2. Trivedi AK, Bararia D, Christopeit M, et al. Proteomic identification of C/EBP-DBD multiprotein complex: JNK1 activates stem cell regulator C/EBPalpha by inhibiting its ubiquitination. Oncogene 2007; 1789-801.
3. Will CL, Schneider C, Hossbach M, et al. The human 18S U11/U12 snRNP contains a set of novel proteins not found in the U2-dependent spliceosome. RNA 2004; 10:929-41.