2X PCR Master Mix

2X PCR Master Mix

Catalog# : NB-28009

Price/Unit : $339.00

Unit : 500 reactions

2x PCR Master Mix

• Convenient ready-to-use solution
• High sensitivity and yield
• Robust amplification
• Available in 3 convenient sizes: 100, 200 and 500 reactions (20 µL vol)
  

Features and Benefits | Specifications

BSI's 2X PCR Master Mix is a ready-to-use solution that contains components required for PCR amplification including Taq DNA polymerase, dNTPs, reaction buffer, MgCl2, KCl and a PCR enhancer/stabilizer. The user needs only to add template, the primer set and water to the master mix in order to set up the PCR reaction. This convenient 2X PCR Master Mix reduces the time required to set up PCR reactions and reduces the possibility of contamination, particularly when preparing large numbers of reactions. The optimized master mix allows for robust amplification of DNA templates with high yields of PCR products.

Taq DNA Polymerase is a highly thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity and a very low 5´→ 3´ exonuclease activity. The source of Taq included with BSI’s 2X PCR Master Mix is an E. coli strain with a cloned Taq DNA Polymerase gene from Thermus aquaticus YT-1.

BSI’s 2X PCR Master Mix is available in 3 convenient sizes:

Cat # NB-28007 - 100 reactions (sufficient for 100 reactions x 20 µL reaction volume)
Cat # NB-28008 - 200 reactions (sufficient for 200 reactions x 20 µL reaction volume)
Cat # NB-28009 – 500 reactions (sufficient for 500 reactions x 20 µL reaction volume)

FEATURES AND BENEFITS

• Convenient - With the ready-to-use Master Mix, the user needs only to add template, the primer set and water to the master mix in order to set up the PCR reaction.
• Time Savings - Set up PCR reactions in a shorter time, as less pipetting steps are required.
• Cost Efficient - No need to buy separate enzymes, dNTPs and buffers. All are included with the ready-to-use Master Mix.
• High Sensitivity and Yield – the optimized Master Mix allows for highly sensitive amplifications with high yields of PCR products.
• Robust Amplification - BSI’s 2X PCR Master Mix is capable of amplifying difficult templates with a high degree of reproducibility.
  

APPLICATIONS AND DATA

• Routine PCR amplification of DNA templates
• PCR for post reverse transcription step
• Multiple band detection or genotyping

Figure 1. Successful Amplification of Different Sized Amplicons. BSI’s 2X PCR Master Mix was used in a series of PCR reactions to amplify a number of different sized amplicons from the adenovirus E2 region. Lanes 1 and 2 correspond to a 189 bp product, Lanes 3 and 4 correspond to a 565 bp product, Lanes 5 and 6 correspond to a 1501 bp product, and Lanes 7 and 8 correspond to a 2202 bp product. BSI’s 2X PCR Master Mix was able to amplify all the different sized products with a high sensitivity and yield. Lane M1 is BSI’s PCR Sizer 100 bp DNA Ladder (Cat #NB-11400) and Lane M2 is BSI’s MidRanger 1kb DNA Ladder (Cat # NB-11700).

SPECIFICATIONS

Figure 1. Highly Stable after Multiple Freeze-Thaw Cycles The stability of BSI’s 2X Master Mix was tested by subjected the mix to multiple freezing and thawing cycles, followed by using the Master Mix to amplify the 16s rDNA (1.5 kb) of Staphylococcus aureus gDNA. BSI’s 2X Master Mix showed robustness and outstanding stability from multiple freezing and thawing steps at -20ºC. M: FastRunner DNA Ladder (Cat#NB-12800). Ten μL of each PCR reaction was loaded on a 1% TAE agarose gel.

Reagents Supplied - Cat # 28007
2X PCR Master Mix (1 Vial, 100 Reactions) Sufficient reagent for 100 x 20 µL reactions

Reagents Supplied – Cat # 28008
2X PCR Master Mix (2 Vials, 100 Reactions each) Sufficient reagent for 200 x 20 µL reactions

Reagents Supplied – Cat # 28009
2X PCR Master Mix (5 Vials, 100 Reactions each) Sufficient reagent for 500 x 20 µL reactions

Storage Conditions and Product Stability
BSI’s 2X Master Mix should be stored at -20ºC. For everyday use an aliquot can be stored at 4ºC for up to three months. The Master Mix is stable for multiple freeze-thaw cycles (see Figure 2). When stored at the proper temperature this reagent is stable for at least 1 year.

Long-Term Storage : BSI’s 2X Master Mix should be stored at -20ºC. For everyday use an aliquot can be stored at 4ºC for up to three months. The Master Mix is stable for multiple freeze-thaw cycles (see Figure 2). When stored at the proper temperature this reagent is stable for at least 1 year.

Product Usage
For laboratory research use only, not for diagnostic use! Not for use in humans.

For more detail regarding Peptides please visit here : Custom Peptide Synthesis