APG7 Antibody

APG7 Antibody

Catalog# : 3615

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2). This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1 (3). Another member of the autophagy family of proteins is APG7 which was identified in yeast as a ubiquitin-E1-like enzyme; this function is conserved in the mammalian homolog (4). In mammalian cells, APG7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles (5). It has been suggested that caspase-8 may alter APG7 levels and thus the APG7 program of autophagic cell death (6).

Additional Names : APG7 (CT), Autophagy protein 7 / ATG7
Description
Left: Western blot analysis of APG7 in Caco-2 cell lysate with APG7 antibody at (A) 0.5, (B) 1 and (C) 2 µg/ml. Below: Immunocytochemistry of APG7 in MCF7 cells with APG7 antibody at 10 µg/ml.

Source : APG7 antibody was raised against a 17 amino acid peptide from near the carboxy terminus of human APG7.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : APG7 antibody was raised in rabbit.

Immunogen : Human APG7 / Autophagy protein 7 / ATG7 (C-Terminus) Peptide (Cat. No. 3615P)

Tested Application(s) : E, WB, ICC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 3615P - APG7 Peptide

Long-Term Storage : APG7 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control

• Cat. No. 1223 - Caco-2 Cell Lysate
• Cat. No. 1219 - MCF7 Cell Lysate

Species Reactivity : H, M

GI Number : 5453668

Accession Number : NP_006386

Short Description : (CT) An autophagy related protein

References

1. Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004; 23:2891-906.
2. Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5.
3. Kamada Y, Funakoshi T, Shintani T, et al. Tor-mediated induction of autophagy via Apg1 protein kinase complex. J. Cell. Biol. 2000; 150:1507-13.
4. Mizushima N, Noda T, Yoshimori T, et al. A protein conjugation system essential for autophagy. Nature 1998; 395:395-8.

For more detail regarding Peptides please visit here : Custom Peptide Synthesis