Catalog# : 3519
Akt1, initially identified as the cellular homolog to the retro-viral oncogene v-Akt (1), is part of the phosphatidyl 3’-kinase (PI3K)-Akt signaling pathway that is activated by diverse cellular stimuli and regulates critical cellular functions such as cell growth, proliferation, and survival (2). Following phosphorylation of the second messenger PIP2 by PI3K, Akt1 translocates to the cell membrane where it is activated by phosphoinositide-dependent kinase (PDK) 1 and PDK2 (3). The active Akt1 is then able to phosphorylate and activate its substrates, including those that are important for cell proliferation and survival such as TOR and the Bcl-2 homolog Bad (4,5). Negative regulation of the PI3K-Akt signaling pathway is mainly accomplished by the lipid phosphatase activity of PTEN which catalyzes the conversion of PIP3 to PIP2, thereby preventing the activation of Akt1. Inactivation of this gene often results in excessive Akt1 activity, often leading to the formation of malignant tumors (reviewed in 6).
Additional Names : Akt1, Protein kinase B / PKB
DescriptionLeft: Western blot analysis of Akt1 in human liver cell lysate with Akt1 antibody at (A) 1 and (B) 2 µg/ml. Below: Immunohistochemistry of Akt1 in human liver cells with Akt1 antibody at 2 µg/ml.
Source : Akt1 antibody was raised against a 16 amino acid peptide from near the amino-terminus of human Akt1.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : Akt1 antibody was raised in rabbit.
Immunogen : Human Akt1 / Protein kinase B / PKB Peptide (Cat. No. 3519P)
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 3519P - Akt1 Peptide
Long-Term Storage : Akt1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1304 - Human Liver Tissue Lysate
GI Number : 12653417
Accession Number : AAH00479
Short Description : a serine/threonine kinase
References
- Bellacosa A, Testa JR, Staal SP, et al. A retroviral oncogene, akt, encoding a serine-threonine kinase containing an SH2-like region. Science 1991; 254:274-7.
- Song G, Ouyang G, and Bao S. The activation of Akt/PKB signaling pathway and cell survival. J. Cell. Mol. Med. 2005; 9:59-71.
- Alessi DR, Adnjelkovic M, Caudwell B, et al. Mechanism of protein kinase B by insulin and IGF-1. EMBO J. 1996; 15:6541-51.
- Nave BT, Ouwens M, Withers DJ, et al. Mammalian target of rapamycin is a direct target for protein kinase B: Identification of a convergence point for opposing effects of insulin and amino-acid deficiency on protein translation. Biochem. J. 1999; 344:427-31.
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