Plasmid DNA Miniprep

Plasmid DNA Miniprep

Catalog# : NB-13300-050

Price/Unit : Inquire

Unit : 50/kit

This Plasmid Miniprep Kit is designed for the rapid purification of plasmid DNA from bacterial cultures. The kit allows for the removal of RNA, SDS, genomic DNA and other contaminants. The kit can also be used to prepare plasmid DNA in a concentrated form. The recovered DNA is of a high quality, and is suitable for a number of downstream applications including digestions, ligations, transformations and sequencing. The kit employs the use of a proprietary resin for DNA purification. This resin is an extremely inert and stable substance that is able to preferentially bind nucleic acids in the presence of high salts and low pH. Elution of the DNA is then dependent on a low salt concentration and higher pH conditions.

Features

• High Recovery: Recovery is 5 to 25µg (depending on copy number)
• Versatile:Plasmids up to 13kb can be purified
• High Column Capacity: Bind up to 25µg of plasmid DNA.
• Fast: Rapid spin-column format allows for the processing of multiple samples in 30 minutes.
• Complete: Kits contain all columns and solutions required

An additional application of the Norgen Plasmid Miniprep Kit is that it can be used as an alternative to cesium chloride banding and ethanol precipitation in order to obtain clean DNA. Simply follow the protocol for the Miniprep to remove contaminants and concentrate your DNA.
Figure 1. High DNA Yield from Small Cultures

Plasmid DNA from bacterial cultures grown overnight was prepared with the Our Plasmid MiniPrep Kit and compared with two other commercial kits. The yield for each of the triplicates is shown on the graph (N1 to N3 for Norgen, and Y1 to Y3 and Z1 to Z3 for competitor kits).
Figure 2. High-Accuracy Sequencing of DNA Product

Sequencing of purified plasmid DNA as a template resulted in an accuracy of 99% over a 950 bp contiguous sequence.Figure 3. Full Compatibility with Restriction Digests

Purified plasmid DNA is highly digestible with restriction enzymes, usually requiring less time to digest completely than DNA purified with other kits as shown in time-course digestion experiments. In Figure 3 digestions were performed with Hind III, and N = Norgen DNA, Y = competitor 1, Z = competitor 2 and C = control DNA.

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