ADAM10 Antibody

ADAM10 Antibody

Catalog# : 2051

Proinflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-alpha, notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF-alpha, notch and its ligand delta (1-3). The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ), and MADM, respectively, (1,2,4). ADAM10 mRNA is expressed in a variety of human and bovine tissues (1,4).

Additional Names : ADAM10 (CT), KUZ

Description

Left: Western blot analysis of ADAM10 in Jurkat whole cell lysate in the absence (1) or presence (2) of blocking peptide (Cat No. 2051P) with ADAM10 antibody at 1 µg/ml. Below: Immunocytochemistry staining of K562 cells using ADAM10 antibody at 2 µg/ml.

Source : ADAM10 antibody was raised against a peptide corresponding to amino acids 732 to 748 of human ADAM10. This sequence is identical to those of bovine and rat origins and differs from that of mouse ADAM10 by one amino acid (2,4).
Purification : Ion exchange chromatography purified Clonality and Clone : This is a polyclonal antibody.

Host : ADAM10 antibody was raised in rabbit.

Immunogen : Human ADAM10 (C-Terminus) Peptide (Cat. No. 2051P)

Application : ADAM10 can be used for detecti

Tested Application(s) : E, WB, ICC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 2051P - ADAM10 Peptide

Long-Term Storage : ADAM10 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control

• Cat. No. 1205 - Jurkat Cell Lysate
  

Species Reactivity :H

GI Number : 4557251

Accession Number : NP_001101

Short Description : (CT) TNFalpha converting enzyme

References

1. Rosendahl MS, Ko SC, Long DL, et al. Identification and characterization of a pro-tumor necrosis factor-alpha-processing enzyme from the ADAM family of zinc metalloproteases. J Biol Chem 1997;272:24588-93
2. Pan D, Rubin GM. Kuzbanian controls proteolytic processing of Notch and mediates lateral inhibition during Drosophila and vertebrate neurogenesis. Cell 1997;90:271-80
3. Qi H, Rand MD, Wu X, Sestan N, Wang W, Rakic P, Xu T, Artavanis-Tsakonas S. Processing of the notch ligand delta by the metalloprotease Kuzbanian. Science 1999;283:91-4
4. Howard L, Lu X, Mitchell S, Griffiths S, Glynn P. Molecular cloning of MADM: a catalytically active mammalian disintegrin-metalloprotease expressed in various cell types. Biochem J 1996;317:45-50. (RD1299)

For more detail regarding Peptide please visit here : Custom Peptide Synthesis