Direct Fungi DNA Isolation Kit

Direct Fungi DNA Isolation Kit

Catalog# : NB-25600

Price/Unit : $224.00

Unit : 25 preps

This Direct Fungi DNA Isolation kit provides a rapid method for the isolation and purification of total DNA from the fungi found on the surface of symptomatic plant samples. The major benefit of this kit is that it does not require the use of physical homogenization steps to isolate the fungal DNA, which are often time consuming and tedious procedures that are not suitable methods for use in the analysis of large numbers of samples. Furthermore, in the area of pathogen diagnosis, simple and convenient sample preparation steps are a critical factor in supporting high throughput analysis. Therefore, this Direct Fungi DNA Isolation Kit adopts a simple and rapid sample preparation procedure that does not rely on physical homogenization steps to isolate sufficient amounts of high quality fungal DNA that can be used in sensitive downstream detection methods.

Features

• Rapid isolation of fungal DNA from plant surfaces
• No liquid nitrogen or physical disruption methods required
• Isolate high quality DNA for a wide range of downstream applications

Direct Fungi DNA Isolation Kit Benefits

• No liquid nitrogen or physical disruption methods required - Rapidly isolate fungal DNA from the surface of symptomatic plant samples without any physical disruption methods.
• Rapid and simple processing - Rapid spin-column format and no physical disruption allows for the processing of multiple samples in 60 minutes.
• High quality fungal DNA - The purified DNA is of the highest quality and can be used in a number of sensitive downstream applications, including PCR.
• Purified DNA can be use in a number of downstream applications - Purified fungal DNA can be used in a number of downstream applications including real time PCR, sequencing, Southern blotting and SNP analysis.

Direct Fungi DNA Isolation Kit Applications

• PCR
• qPCR
• sequencing
• Southern blotting
• SNP analysis

Figure 1. Isolation and Identification of Fungal Pathogens from Grapes

DNA was isolated from the surface of symptomatic grapes using the Direct Fungi DNA Isolation Kit. The DNA was subsequently used as the template in end-point PCR reactions using universal primers for the fungal ITS region. The PCR products are shown on the 1% agarose gel above. Through sequencing it was determined that DNA was successfully isolated and amplified from 7 different fungal species including A. niger (Lane 1), C. cladosporiodes (Lane 2), B. cinerea (Lane 3), M. racemosus (Lane 4), A. tenuissima (Lane 5), Penecillium sp. (Lane 5), F. oxysporum (Lane 6) and R. oryzae (Lane 7). Lane C is the non-template control and Lane M is Bisynthesis PCRSizer DNA Ladder.
Figure 2. Screening for Botrytis cinerea Infection

Fungal DNA was isolated from the surface of 5 varieties of symptomatic grapes using the Direct Fungi DNA Isolation Kit. The DNA was then used in a qPCR reaction for the detection of Botrytis cinerea infection . From observing the graph above it can be seen that Botrytis infection can be found on both the Cabernet Sauvignon and the Voigner grapes.
Long-Term Storage : Room Temperature

Product Usage : For laboratory research use only, not for diagnostic use! Not for use in humans.

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