Saliva DNA Isolation

Saliva DNA Isolation

Catalog# : NB-21410

Price/Unit : $168.00

Unit : 25 preps

Human genomic DNA found in saliva can be used in various applications in diagnostics including the detection of biomarkers to diagnose a disease, follow the disease’s progress or monitor the effects of a particular treatment. Isolation of DNA from saliva for diagnostics has become an attractive alternative to isolation from blood or tissue due to the fact that sample collection is non-invasive.

This Saliva DNA Isolation Kit provides a fast and simple procedure for isolating genomic DNA from up to 500 uL of saliva. The Preservation Solution provided with the kit allows for the storage of the saliva samples for extended periods of time at room temperature prior to DNA isolation. Purification is based on spin column chromatography using this proprietary resin as the separation matrix . The genomic DNA is preferentially purified from the other cellular components such as RNA and proteins. The purified saliva DNA is compatible with a number of downstream applications including PCR, Southern Blot analysis, sequencing and microarray analysis.

Features

• Isolate and detect DNA from as little as 0.1 mL of saliva
• High yield
• Fast and efficient

Saliva DNA Isolation Kit Benefits

• Fast and easy processing- Rapid spin-column format allows for the processing of 10 samples in 30 minutes.
• Sample collection is non-invasive and painless- Saliva offers an attractive alternative to blood or tissue for the isolation of human DNA samples for use in diagnostic applications.
• Isolate DNA from small saliva samples - DNA can be isolated and detected from as little as 100 µL of saliva.
• Recovered DNA is suitable for various downstream applications - Purified saliva DNA can be used in a number of downstream applications including PCR, Southern Blot analysis, sequencing and microarray analysis.
  

Applications

• PCR
• Southern Blot Analysis
• Sequencing
• Microarray Analysis

Figure 1. Isolate High Quality Saliva DNA

DNA was isolated from three different 1 mL saliva samples using Biosynthesis Saliva DNA Isolation Kit (Lanes A-C) and run on a 1.8% agarose gel. Note the high yield and quality of the DNA in all lanes. Lane M is Biosynthesis MidRanger 1kb DNA Ladder.
Figure 2. Isolated Saliva DNA can be used in PCR Reactions

Saliva DNA isolated from 3 different individuals was used as the template in a PCR reaction to amplify the K-ras gene. Lanes A - C contain the expected 157 bp product, and correspond to 3 different saliva DNA samples. Lane D is the negative control and shows only primer dimers, while Lane E is the positive control of 293 HEK DNA, and again shows the expected 157 bp product. Lane M is Biosynthesis FastRunner DNA Ladder.
Product Usage

For laboratory research use only, not for diagnostic use! Not for use in humans.

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