Thursday, March 11, 2010

Genomic DNA Isolation Kit

Genomic DNA Isolation Kit

Catalog# : NB-24700

Price/Unit : $168.00

Unit : 50 preps

This Genomic DNA Isolation Kit is designed for the rapid preparation of genomic DNA from various tissue samples, cultured cells, bodily fluids, and viruses. Purification is based on spin column chromatography using Biosynthesis patented resin as the separation matrix. This resin binds DNA under high salt concentrations and releases the bound DNA under low salt and slightly alkali conditions. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with PCR and Southern Blot analysis.

Features
  • Isolate genomic DNA from animal tissues, cells, bodily fluids and swabs
  • Rapid and convenient spin column procedure
  • Purified DNA is of the highest quality and integrity for sensitive downstream applications
Genomic DNA Isolation Kit Benefits
  • Fast and easy processing - Rapid spin-column format allows for the processing of multiple samples in 80 minutes (including a 1 hour incubation)
  • High binding capacity of columns - The binding capacity of the columns in the Genomic DNA Isolation Kit is 25 ug
  • Isolate genomic DNA from a diversity of species - Genomic DNA can be isolated from various tissue samples, cultured cells, bodily fluids including blood and saliva, and viruses
  • Recovered genomic DNA is suitable for downstream applications - Purified genomic DNA is fully compatible with restriction enzyme digestions, PCR amplifications and Southern Blot analysis.
  • High quality DNA - No degradation of the genomic DNA isolated with the Genomic DNA Isolation Kit is observed.
Applications
  • Restriction Enzyme Digestions
  • PCR
  • Quantitative PCR
  • Southern Blot Analysis

Figure 1. Isolation of High Quality Genomic DNA
Genomic DNA was isolated from various sample types using this Genomic DNA Isolation Kit. Lanes 1-4 contain genomic DNA that was isolated from 4 different samples containing 5 x 10^5 HeLa cells, while lanes 5 and 6 contain genomic DNA that was isolated from 5 mg of heart tissue. All the purified genomic is of the highest quality and integrity.
Figure 2. Full Compatibility with Digests
Genomic DNA was isolated from 10 mg of kidney tissue using this Genomic DNA Isolation Kit, and subsequently digested. Each Lane 1 contains undigested Biosynthesis-isolated DNA, while Lanes 2 contain the genomic DNA digested with Hind III and Lanes 3 contain the genomic DNA digested with Nde I. Lane M is Biosynthesis HighRanger 1kb DNA Ladder.

Product Usage
For laboratory research use only, not for diagnostic use! Not for use in humans.

For more detail regarding Peptides please visit here : Custom Peptide Synthesis

1 comment:

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