Catalog# : 3165
Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain (reviewed in 1 and 2). BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bcl-G (3). The mRNA of Bcl-G encodes 2 isoforms, Bcl-GL, which is widely expressed in multiple tissues, and Bcl-GS, which is only found in testis. The Bcl-GS protein is predominantly localized to cytoplasmic organelles whereas Bcl-GL was distributed throughout the cytosol. Overexpression of either protein induced apoptosis, although Bcl-GS was far more potent than Bcl-GS. Apoptosis induction was dependent on the BH3 domain and could be suppressed by co-expression with the anti-apoptotic Bcl-XL protein (3)
Additional Names : Bcl-G (CT), Bcl-2-like 14
DescriptionLeft: Western blot analysis of Bcl-G in U937 cell lysates with Bcl-G antibody at (A) 2.5 and (B) 5 µg/ml. Below: Immunohistochemical staining of mouse testis tissue using Bcl-G antibody at 2 µg/ml.
Source : Bcl-G antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of human Bcl-G.
Purification : Ion exchange chromatography purified
Clonality and Clone : This is a polyclonal antibody.
Host : Bcl-G antibody was raised in rabbit.
Immunogen : Murine Bcl-G / Bcl-2-like 14 (C-Terminus) Peptide (Cat. No. 3165P)
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 3165P - Bcl-G Peptide
Long-Term Storage : Bcl-G antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1215 - U937 Cell Lysate
- Cat. No. 1416 - Mouse Testis Tissue Lysate
GI Number : 13540528
Accession Number : NM_030766
Short Description : (CT) Bcl-2 family member
References
- Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607.
- Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron.. 2004; 39:1125-35.
- Guo B, Godzik A, and Reed JC. Bcl-G, a novel pro-apoptotic member of the Bcl-2 family. J. Biol. Chem. 2000; 276:2780-5.
1 comment:
Inhibitor peptide corresponding to last 10 amino acids of the C-terminus of the GluA2 AMPA receptor subunit that inhititss binding of GluA2 (at the C-terminal PDZ site) to glutamate receptor interacting protein (GRIP), AMPA receptor binding protein (ABP) and protein interacting with C kinase (PICK1). Increases AMPA receptor-mediated currents amplitude and blocks long-term depression (LTD). pep2-SVKI
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