Catalog# : NB-22300
Price/Unit : $196.00
Unit : 20 preps
This Urine (Exfoliated Cell) DNA Isolation Kit is designed for the rapid preparation of genomic DNA from exfoliated cells that have been shed into the urine from the urinary tract. The genomic DNA isolated from these exfoliated cells can be used in a number of research applications including biomarker studies for the diagnosis and monitoring of bladder, kidney, or other urinary-tract cancers. Purification is based on spin column chromatography using this proprietary resin as the separation matrix. The kit preferentially purifies DNA from other cellular proteinaceous components, as well as from the contaminating species found in urine such as glucose and salts.
Features
- Isolate and detect genomic DNA from as little as 1 mL of urine
- Provides high quality DNA for sensitive applications
- Rapid processing time from sampling to downstream testing
- Fast processing - Rapid spin-column format allows for the processing of multiple samples in 45 minutes.
- Isolate genomic DNA from small volumes of urine- DNA can be isolated and detected from the exfoliated cells found in as little as 1 mL of urine.
- High quality DNA - Removal of highly concentrated salts, metabolic wastes and proteins provides high quality DNA to be used in various downstream applications.
- Recovered DNA is suitable for downstream applications - Purified DNA is fully compatible with PCR, quantitative PCR and Southern Blot analysis.
- PCR
- Quantitative PCR
- Southern Blot Analysis
Figure 1. Isolation and Detection of Biologically Active Genomic DNA from 1 mL of UrineGenomic DNA was isolated from the exfoliated cells found in a 1 mL urine sample using this Urine (Exfoliated Cell) DNA Isolation Kit. The isolated DNA was then subjected to quantitative PCR using human beta actin gene primers to detect the genomic DNA. The red lines in the PCR baseline graph above correspond to DNA standards, while the blue lines correspond to the successful PCR results when DNA isolated from the exfoliated cells in 1 mL of urine was used as the template.
Product UsageFor laboratory research use only, not for diagnostic use! Not for use in humans.
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