Abundant Serum Protien Depletion Kit

Abundant Serum Protien Depletion Kit

Catalog# : NB-17300
Price/Unit : $220.00
Unit : 25 preps

This Depletion Kit provides a fast and simple procedure for the effective depletion of major serum proteins including albumin , alpha -antitrypsin, transferin and haptoglobin from serum and plasma samples. The kit is unique in that it is based on an ion-exchange mechanism and not the use of specific antibodies. As a result, the kit can be used to deplete serum proteins from a wide variety of samples, including human and various animals.

Each spin column can deplete up to 500 µg of serum proteins. Albumin has been found to be depleted by 70%, transferrin and haptoglobin by 50% and alpha -antitrypsin by 90%. The complexity of the sample is thus greatly reduced, allowing for the detection of less abundant proteins present in the sample. Eluted samples are ready for use in various downstream applications including 2D gel electrophoresis, LC/MS and microarrays.

ProteoSpin™ Abundant Serum Protein Depletion Kit Benefits

• Efficient removal of highly abundant proteins - The kit depletes 70% of albumin, 90% of alpha-antitrypsin, and 50% of transferrin and haptoglobin, enabling the visualization of low abundance proteins..
• Fast and easy processing - Efficiently process up to 10 samples in less than 30 minutes using an easy-to-use protocol.
• Does not rely on antibodies - Based on an ion exchange mechanism, the kit can be applied to serum and plasma samples from a range of sources, including human and various animals.
• Complete Kit -All columns and solutions required are provided.
• Low price per column - Compared to columns based on antibodies, these ion-exchange columns are much lower in price, yet perform to the same high standards.
• Depletion of proteins and salts - Most proteins bind to the column, while salts and many of the major proteins are removed in the flowthrough.
• Suitable for downstream applications - Final elution suitable for:
• SDS-PAGE
• 2D gel electrophoresis
• LC/MS
• Protein

ProteoSpin™ Abundant Serum Protein Depletion Kit Contents

Each ProteoSpin™ Abundant Serum Protein Depletion Kit provides essential components for use with the provided protocols.

• Column Activation and Wash Buffer
• Elution Buffer
• Neutralizer
• Mini Spin Columns
• Elution Tubes
• Short Protocol Card
• Application Manual

Customer-supplied Reagents and Equipment

• Benchtop microcentrifuge
• Micropipettors

Figure 1. Abundant proteins are depleted from human serum to a large degree using the ProteoSpin™ Abundant Serum Protein Depletion Kit. Lane A represents the input human serum proteins, while Lanes C and D represent the elution after the abundant proteins were depleted with the kit. Lane B represents the binding flowthrough, and contains the proteins that have been depleted from the serum sample.

Figure 2. A 2D gel was run after the abundant serum proteins were depleted from human serum using the ProteoSpin™ Abundant Serum Protein Depletion Kit. The abundant proteins including albumin, alpha-antitrypsin, transferrin and haptoglobin have been removed from the sample, allowing the visualization and detection of the lower abundance proteins of interest.

Product Usage
For laboratory research use only, not for diagnostic use! Not for use in humans.

For more detail regarding Peptide please visit here : Custom Peptide Synthesis