Acinus Antibody

Acinus Antibody

Catalog# : 2219

Chromatin condensation and nuclear fragmentation (CCNF) is the hallmark of apoptosis. CCNF is triggered by the activation of members of caspase family, caspase activated DNase (CAD/DFF40), and several novel proteins including AIF and CIDE . A new inducer of chromatin condensation was recently identified and designated Acinus (for apoptotic chromatin condensation inducer in the nucleus). Acinus is cleaved by caspase-3 and an additional unknown protease generating a small active peptide p17, which causes chromatin condensation in vitro when it is added to purified nuclei. Acinus also induces apoptotic chromatin condensation in cells. Acinus is ubiquitously expressed. Three different spliced forms of Acinus have been identified in human and mouse and designated AcinusL, AcinusS and AcinusS’ (2).

Additional Names : Acinus (IN), Acinus

Description
Left: Western blot analysis of Acinus in K562 whole cell lysate with Acinus antibody (IN) at 1 µg/ml. Below: Immunocytochemistry of Acinus in K562 cells with Acinus antibody at 5 µg/ml.


Source : Acinus antibody was raised against a peptide (dtsenrpendvpepp) corresponding to amino acids 775 to 789 of human AcinusL, 48 to 62 of human AcinusS’, or 17 to 31 of human AcinusS, which differ from those of mouse Acinus by one amino acid.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : Acinus antibody was raised in rabbit.

Immunogen : Murine Acinus (Intermediate Domain) Peptide (Cat. No. 2219P)

Application : Acinus antibody can be used for
Tested Application(s) : E, WB, ICC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 2219P - Acinus Peptide

Long-Term Storage : Acinus antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control

1. Cat. No. 1204 - K562 Cell Lysate

Species Reactivity : H

GI Number : 5931959

Accession Number : AAD56724

Short Description : (IN) Inducer of chromatin condensation

References

1. Zamzami N, Kroemer G. Condensed matter in cell death. Nature 1999 ;401:127-8.
2. Sahara S, Aoto M, Eguchi Y, Imamoto N, Yoneda Y, Tsujimoto Y. Acinus is a caspase-3-activated protein required for apoptotic chromatin condensation. Nature 1999 401:168-73. (WD0800