Urine DNA Isolation Kit

Urine DNA Isolation Kit

Catalog# : NB-18100

Price/Unit : $385.00

Unit : 50 preps

This Urine DNA Isolation Kit provides a fast and simple procedure for isolating DNA from 2 mL of urine. DNA found in urine can be divided into 2 basic categories. The larger species is generally greater than 1 kb in size, and appears to derive mainly from cells shed into the urine from the urinary tract. The second species is smaller, generally between 150 and 250 bp, and derives at least in part from the circulation. Both types of urine DNA can be isolated using this kit.

With this Urine DNA Isolation Kit, purification is based on spin column chromatography using Biosynthesis proprietary resin as the separation matrix. The kit allows for the removal of highly concentrated salts, metabolic wastes and proteins, to allow for the isolation of high quality, concentrated DNA. The purified urine DNA is compatible with PCR and Southern Blot analysis.

Features and Benefits:

• Fast and easy processing: Rapid spin-column format allows for the processing of multiple samples in 40 minutes.
• Isolation of both types of urine DNA: Isolate both high molecular weight DNA (greater than 1 kb in size; mostly cell associated) and the smaller DNA (150 - 250 bp; derived from the circulation).
• High quality DNA: Removal of highly concentrated salts, metabolic wastes and proteins provides high quality, concentrated DNA to be used in various downstream applications.
• Recovered DNA is suitable for downstream applications: Purified DNA is fully compatible with PCR analysis and Southern Blot analysis.
• No mixing or formulation: Solutions are provided for with no need for formulation.

Figure 1. Agarose gel of DNA Isolated using this Urine DNA Isolation Kit Urine DNA was isolated from two different male samples using this kit. The DNA was then run on a 2% agarose gel. As can be seen from the gel picture, the kit is isolating both the larger and smaller species of urine DNA. Lane M is marker, while Lanes S1 and S2 correspond to the two different DNA samples.
Figure 2. Isolated Urine DNA can be used as the Template in PCR Reactions. DNA was isolated from the urine of a human male using this kit. The DNA was then used as the template in a nested PCR reaction to detect Ychromosome- specific DNA sequences. Lane A is the 154 bp fragment that resulted from the first round of PCR, while Lane B contains the 154 bp fragment as well as the 77 bp nested fragment.

Product Usage
For laboratory research use only, not for diagnostic use! Not for use in humans.

For more detail regarding Peptides please visit here : Custom Peptide Synthesis