Catalog# : 3023
Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer (reviewed in 1). Members of the Bcl-2 family are known to be critical regulators of this process. These proteins are characterized by the presence of several conserved motifs termed Bcl-2 homology (BH) domains (reviewed in 2 and 3). A novel, widely expressed member termed Bcl-rambo was recently identified. This protein is localized to mitochondria in mammalian cells and its overexpression induces apoptosis which could be blocked by co-expression of inhibitor of apoptosis proteins (IAPs) such as XIAP, cIAP1, and cIAP2 (4). Bcl-rambo shows overall homology to the anti-apoptotic members containing BH motifs, but unlike Bcl-2, the C-terminal membrane anchor of Bcl-rambo is preceded by a unique 250 amino acid insertion. This region by itself can induce apoptosis more efficiently than the Bcl-2 homology regions, suggesting that Bcl-rambo may be important other pro-apoptotic pathways (4).
Additional Names : Bcl-rambo (IN), Bcl-2 like protein 13 / Mil1
DescriptionLeft: Western blot analysis of Bcl-rambo in K562 cell lysate with Bcl-rambo antibody at (A) 2 and (B) 4 µg/ml. Below: Immunohistochemistry of Bcl-rambo in human heart tissue with Bcl-rambo antibody at 10 µg/ml.
Source : Bcl-rambo antibody was raised against a 15 amino acid peptide from near the center of human Bcl-rambo.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : Bcl-rambo antibody was raised in rabbit.
Immunogen : Human Bcl-rambo / Bcl-2 like protein 13 / Mil1 (Intermediate Domain) Peptide (Cat. No. 3023P)
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 3023P - Bcl-rambo Peptide
Long-Term Storage : Bcl-rambo antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1204 - K562 Cell Lysate
- Cat. No. 1301 - Human Heart Tissue Lysate
GI Number : 14043326
Accession Number : AAH07658
Short Description : (IN) a Bcl-2 protein family member
References
- Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.
- Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607.
- Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron. 2004; 39:1125-35.
- Kataoka T, Holler N, Michau O, et al. Bcl-rambo, a novel Bcl-2 homologue that induces apoptosis via its unique C-terminal extension. J. Biol. Chem. 2001; 276:19548-54.,
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