Fungi/Yeast Genomic DNA Isolation Kit

Fungi/Yeast Genomic DNA Isolation Kit

Catalog# : NB-27300

Price/Unit : $196.00

Unit : 50 preps

This Fungi/Yeast Genomic DNA Isolation Kit is designed for the rapid preparation of genomic DNA from viable yeast cells, fungal spores or mycelium and Gram-positive bacteria. Genomic DNA is efficiently extracted from the cells by a combination of heat treatment, detergents and the use of provided Bead Tubes. Purification is based on spin column chromatography using this proprietary resin as the separation matrix. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with downstream applications such as PCR, Restriction Fragment Length Polymorphism (RFLP) and Amplified Fragment Length Polymorphism (AFLP) . Typical yields of genomic DNA will vary depending on the cell density of the yeast or fungal culture and species. The option of an additional lyticase treatment is also provided in order to allow for improved DNA yields for certain fungal and yeast species.

Features

• Convenient enzyme-free bead beating protocol
• Purified DNA fully compatible with sensitive downstream applications
• Rapid and simple procedure

Fungi/Yeast DNA Isolation Kit Benefits

• Isolate DNA from a wide range of samples - Genomic DNA can be isolated from viable yeast cells, fungal spores or mycelium and Gram-positive bacteria.
• No phenol :chloroform extractions - DNA is isolated without the use of harmful chemicals such as phenol or chloroform.
• Rapid and simple processing- Rapid bead-beating and spin-column format allows for the processing of multiple samples in 45 minutes.
• Isolate high quality DNA - Purified DNA is of the highest quality and can be used in a number of downstream applications including PCR, Restriction Fragment Length Polymorphism (RFLP) and Amplified Fragment Length Polymorphism (AFLP) .

Fungi/Yeast Genomic DNA Isolation Kit Applications

• PCR
• Restriction Fragment Length Polymorphism (RFLP)
• Amplified Fragment Length Polymorphism (AFLP)

Figure 1. Isolate DNA from a Wide Range of Samples
DNA was isolated from 50 mg (wet weight) samples of Pichia sp. (Lane 1), Aspergillus niger (Lane 2), Cladosporium cladasporioides (Lane 3), Botrytis cinerea (Lane 4) and Mucor racemosus (Lane 5) using this Fungi/Yeast Genomic DNA Isolation Kit, and 5 µL aliquots were run on a 1X TAE 1% agarose gel. As it can be seen, high quality DNA was isolated in all cases. The M Lane contains Biosynthesis HighRanger 1kb DNA Ladder.
Figure 2. Purified DNA can be Amplified in a qPCR Reaction

DNA was isolated from 50 mg samples of Pichia sp. , Aspergillus niger , Cladosporium cladasporioides , Botrytis cinerea and Mucor racemosus using this Fungi/Yeast Genomic DNA Isolation Kit, and 2 µL of the DNA was used in a qPCR (SYBR Green) reaction with specific fungal and yeast primers. The qPCR was successful in amplifying and detecting all the yeast and fungal DNA, indicating that the DNA is of a high quality and can be used in sensitive downstream applications.

Product Usage
For laboratory research use only, not for diagnostic use! Not for use in humans.

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