DNA Gel Extraction Kit

DNA Gel Extraction Kit

Catalog# : NB-13100

Price/Unit : $125.00

Unit : 50 preps

The DNA Gel Extraction Kit is designed for the rapid purification of DNA fragments from agarose gels. The kit allows for the removal of agarose, enzymes, dNTPs, ethidium bromide, dyes and detergents from the DNA fragment. The recovered DNA is of a high quality, and is suitable for a number of downstream applications including restriction enzyme digestions, ligations, labeling, hybridizations and sequencing.

The kit employs the use of a proprietary resin for DNA purification. This resin is an extremely inert and stable substance and is able to preferentially bind nucleic acids in the presence of high salts and low pH. Elution of the DNA is then dependent on a low salt concentration and higher pH conditions

Features and Benefits:

• High Yield: Recovery is up to 90% for inputs of >1 ug.
• Versatile: Purify DNA fragments from 50 bp to 10 kb
• Effective: Purified DNA performs well in downstream applications
• High Column Capacity: Bind up to 10µg of DNA
• Fast: 15 minutes to complete procedure
• Complete: Kits contain all columns and solutions required

Figure 1. Efficient Recovery from Agarose Gels

A 500 bp fragment was purified from a 1% agarose gel using this DNA Gel Extraction Kit (N) and two other commercial kits (Y and Z). This kit shows a significantly higher efficiency compared to the competitor kits.
Figure 2. Broad Size Range of Target DNA

A 261 bp fragment (lane C1), a 630 bp fragment (lane C2) and a 1,058 bp fragment (lane C3) were recovered using both Biosynthesis kit (B1-B3) and a competitor’s kit (Y1-Y3).
Figure 3. Full Compatibility with Restriction Digests

In order to demonstrate the purity of gel extracted DNA fragments, this DNA Gel Extraction Kit was used to purify a 1,200 bp PCR product, which was subsequently digested with Bam HI (lane a), Hind III (lane B) and Pvu II (lane C).

For more detail regarding Peptide please visit here : Custom Peptide Synthesis