PCR Purification Kit

PCR Purification Kit

Catalog# : NB-14400

Price/Unit : $125.00

Unit : 50 preps

This PCR Purification Kit is designed for the rapid purification of PCR products from reaction mixtures. The kit allows for the removal of mineral oil, enzymes, unincorporated nucleotides, primers and salts. Up to 95% of primer dimers are also removed with the kit. The recovered DNA is of a high quality, and is suitable for a number of downstream applications including restriction enzyme digestions, ligations, labeling, hybridizations and sequencing.

The kit employs the use of a novel resin for DNA purification. This resin is an extremely inert and stable substance which is able to preferentially bind nucleic acids in the presence of high salts and low pH. Elution of the DNA is then dependent on a low salt concentration and higher pH conditions

Features and Benefits:

High Yield: PCR Recovery is up to 95%
Versatile: Purify PCR products from 100 bp to 10 kb
Effective: Remove up to 95% of primer dimers
High Column Capacity: Bind up to 10µg of PCR
Fast: 15 minutes to complete procedure
Complete: Kits contain all columns and solutions required

An additional application of this PCR Purification Kit is that it can be used as an alternative to phenol extraction and ethanol precipitation in order to clean up various enzymatic reactions. Enzymes, buffers, dNTPs and other contaminants can be removed from the DNA using this kit as well.
Figure 1. High Yield Purification

The high yield of this PCR Purification Kit is illustrated by purifying a 500 bp PCR-amplified product in a reaction mixture and comparing the recovery with a competitor. The results are shown in Figure 1, which depicts the yields of triplicates (B1to B3 for Biosynthesis, and Y1 to Y3 for the competitor).
Figure 2. Removal of Primer-Dimers

Primer-dimers present in PCR reactions are effectively removed during the purification process with the Norgen PCR Purification Kit. An additional wash with guanidine hydrochloride is added in the procedure to remove greater than 85% of primer dimers. The figure shows alternating lanes containing unprocessed (U) and processed (P) PCR reaction products.

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