Catalog# : 3337
Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer (reviewed in 1). Bcl-2 is the founding member of a family of over 20 proteins that are critical regulators of apoptosis (reviewed in 2 and 3). These can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Bcl-2 is an inner mitochondrial membrane protein that inhibits apoptosis (4). It is thought to act by interacting with pro-apoptotic Bcl-2 family members such as Bak and Bad (5 for review). Overexpression of Bcl-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer (4,6).
Additional Names : Bcl-2 (IN), Bcl-2
DescriptionLeft: Western blot analysis of Bcl-2 in A-20 cell lysates with Bcl-2 antibody at (A) 1, (B) 2, and (C) 4 µg/ml. Below: Immunocytochemistry of Bcl-2 in A20 cells with Bcl-2 antibody at 2 µg/ml.
Source : Bcl-2 antibody was raised against a peptide corresponding to 14 amino acids near the middle of human Bcl-2.
Purification : Ion exchange chromatography purified.
Clonality and Clone : This is a polyclonal antibody.
Host : Bcl-2 antibody was raised in rabbit.
Immunogen : Murine Bcl-2 (Intermediate Domain) Peptide (Cat. No. 3337P)
Tested Application(s) : E, WB, ICC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 3337P - Bcl-2 Peptide
Long-Term Storage : Bcl-2 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1288 - A20 Cell Lysate
GI Number : 20072668
Accession Number : AAH27258
Short Description : (IN) a member of the bcl-2 family
References
- Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.
- Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607.
- Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron. 2004; 39:1125-35.
- Hockenbery D, Nunez G, Milliman C, et al. Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death. Nature 1990; 348:334-6.
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