Urine Bacterial DNA Isolation Kit

Urine Bacterial DNA Isolation Kit

Catalog# : NB-22400

Price/Unit : $196.00

Unit : 20 preps

This Urine Bacteria Genomic DNA Isolation Kit provides a rapid method for the isolation and purification of bacterial genomic DNA from 1 to 20 mL of urine. Bacterial genomic DNA can be isolated from both human urine samples and urine samples from animals in order to study the levels and types of bacteria that are present. The kit allows for the isolation of genomic DNA from both Gram negative and Gram positive bacteria, including E. coli , Proteus spp., Klebsiella spp., Enterobacter spp., Serratia spp., Pseudomonas spp, Clostridial ssp. and Leptospirosis spp., as well as Chlamydia trachomatis and Neisseria gonorrhoeae . Typical yields of genomic DNA will vary depending on the urine sample and the bacterial species, if any, present in the urine. Healthy humans generally have < style="font-weight: bold;">Features

• Isolate and detect bacterial DNA from as little as 1 mL of healthy human urine
• Isolate genomic DNA from both gram-negative and gram-positive bacteria in urine
• Fast and Efficient

Urine Bacteria DNA Isolation Kit Benefits

• Fast and easy processing - Rapid spin-column format allows for the processing of 10 samples in 45 minutes.
• Isolate high quality DNA- No degradation of the genomic DNA isolated with the Urine Bacteria Genomic DNA Isolation Kit is observed.
• Isolate genomic DNA from all types of bacteria found in urine - Bacterial genomic DNA can be isolated from both gram negative and gram positive bacteria using the kit.
• High sensitivity - Isolate and detect bacterial DNA from as little as 1 mL of healthy human urine.
• Recovered genomic DNA is suitable for downstream applications - Purified genomic DNA can be used in a number of downstream applications including restriction enzyme digestions, PCR amplifications, quantitative PCR and Southern Blot analysis.

Applications

• Restriction Enzyme Digestions
• PCR
• Quantitative PCR
• Southern Blot Analysis

Figure 1. Isolation of Genomic DNA from both Gram Positive and Gram Negative Urine Bacteria

This Urine Bacteria Genomic DNA Isolation Kit was used to isolate genomic DNA from urine samples spiked with the Gram positive bacteria B. cereus (Lanes 1 and 2) and the Gram negative bacteria E. coli (Lanes 3 and 4). Lane M is Biosynthesis MidRanger 1kb DNA Ladder.
Figure 2. Isolation and Detection of Bacterial Genomic DNA from 1 mL of Urine

Bacterial genomic DNA was isolated from a 1 mL sample of healthy human urine using the Urine Bacteria Genomic DNA Isolation Kit, and was subsequently detected using quantitative PCR. Healthy humans generally have < onblur="try {parent.deselectBloggerImageGracefully();} catch(e) {}" href="http://www.biosyn.com/images/userfiles/image/4%282%29.jpg">Product Usage

For laboratory research use only, not for diagnostic use! Not for use in humans.

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