Low Copy Plasmic DNA MiniPrep

Low Copy Plasmic DNA MiniPrep

Catalog# : NB-17800

Price/Unit : $110.00

Unit : 25 preps

This Low Copy Plasmid MiniPrep Kit is designed for the rapid preparation of low copy number plasmid DNA from small batches of E. coli. Purification is based on spin column chromatography using this proprietary resin as the separation matrix. This resin binds DNA under high salt concentrations and releases the bound DNA under low salt and slightly alkali conditions. The process does not require ethanol or other alcohols, a significant improvement over other techniques to achieve full compatibility with alcohol sensitive applications. The purified DNA is free of genomic DNA, RNA and cellular proteins and is suitable for many downstream applications. The Low Copy Plasmid MiniPrep Kit contains sufficient materials for 25 preparations. Typical yield from a 3 mL culture is 0.5 - 1 µg. Purification of plasmids up to 14,000 bp in size have been verified.

Features

• High Recovery: 0.5 to 1 µg of low copy DNA can be purified from 3.0 mL of bacterial culture.
• Alcohol-free protocol: No alchohols are used durig the purification process, thus the DNA is compatible with alcohol sensitive downstream applications.
• Fast and Easy Processing: Rapid spin-column format allows for the processing of multiple samples in 30 minutes.
• Recovered DNA is suitable for downstream applications: Purified plasmid DNA is fully compatible with restriction enzyme digestions and sequencing.
  

Table 1. High DNA Yield from Small Cultures

The Low Copy Plasmid MiniPrep Kit was used to isolate two different low copy plasmids from 3 mL cultures. In each case, the isolation was performed in triplicates. The average yield of the low copy number plasmids is high in both cases.

Figure 1. Isolation of High Quality DNA from Low Copy Number Plasmids. The Low Copy Number Plamsid MiniPrep Kit was used to isolate plasmid DNA from 3 mL cultures.

Lanes 1-6 are pSYX20 DNA, while lanes 7-12 are pSC101DNA. Five microlitres of the total elutions were loaded onto the gel, with Lanes 1-3 and 7-9 corresponding to 30 µL elutions, and Lanes 4-6 and 10-12 corresponding to 50 µL elutions. In all cases, the DNA is of a high quality, indicated by the presence of sharp bands and no degradation of the isolated DNA.

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