Catalog# : NB-26200
Price/Unit : $224.00
Unit : 50 preps
This Plant/Fungi DNA Isolation Kit provides a rapid method for the isolation and purification of total DNA from a wide range of plant and filamentous fungal species. Total DNA, including genomic DNA, mitochondrial DNA and chloroplast DNA can be purified from fresh or frozen plant tissues, plant cells or filamentous fungi samples using this kit. The procedure is rapid and convenient, as it does not rely on the use of liquid nitrogen in order to homogenize the samples. The DNA is preferentially purified from other cellular components, such as proteins, without the use of phenol or chloroform. The purified DNA is of the highest integrity, and can be used in a number of downstream applications including real - time PCR, SNP, Southern blotting and sequencing.
Features
- No liquid nitrogen required for homogenization
- Isolate total DNA including viral DNA
- rapid and simple procedure
- No liquid nitrogen required for homogenization - Liquid nitrogen is not required for homogenization of samples, making DNA purification rapid and convenient.
- Isolate DNA from a wide range of samples - DNA can be isolated from a wide range of plant and filamentous fungi samples.
- Isolate total DNA, including viral DNA - Purified DNA samples can be used for the detection of viral pathogens, as viral DNA is isolated with the plant/fungi DNA.
- Rapid and simple processing - Rapid spin-column format allows for the processing of multiple samples in 45 minutes.
- High yield of DNA - High yields of purified DNA can be isolated with this kit.
- No phenol :chloroform extractions - DNA is isolated without the use of harmful chemicals such as phenol or chloroform. The DNA is of the highest quality and can be used in a number of downstream applications.
- qPCR
- Southern blotting
- SNP analysis
- Sequencing
Figure 1. Isolate DNA from a Wide Range of Plants
DNA was isolated from 50 mg samples of tobacco leaves (Lanes 1 and 2), tomato leaves (Lanes 3 and 4), peach leaves (Lanes 5 and 6) and grape leaves (Lanes 7 and 8) using this Plant/Fungi DNA Isolation Kit, and 5 uL aliquots were run on a 1X TAE 1 % agarose gel. As it can be seen, high quality DNA was isolated in all cases. High quality DNA isolated in all cases. The M Lanes contain Biosynthesis HighRanger 1kb DNA Ladder.
Figure 2. Purified DNA Can be Amplified in a qPCR ReactionDNA was isolated from 50 mg samples of plum and tobacco leaves using this Plant/Fungi DNA Isolation Kit, and 2 uL of the DNA was used in a qPCR reaction with 18 srDNA primers. The qPCR was successful in amplifying both the plum and tobacco DNA, indicating that the DNA is of a high quality and can be used in sensitive downstream applications.
Product UsageFor laboratory research use only, not for diagnostic use! Not for use in humans.
For more detail regarding Peptides please visit here : Custom Peptide Synthesis
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