Enzymatic Reaction Clean-up

Enzymatic Reaction Clean-up

Catalog# : NB-19900

Price/Unit : $125.00

Unit : 50 preps

This Enzymatic Reaction Clean-Up Kit enables the rapid purification of DNA from various enzymatic reaction mixes. This kit is able to purify DNA from different enzymatic reactions including restriction enzyme digests, Klenow reactions, alkaline phosphatase reactions, and ligations. Purification is based on spin column chromatography using Biosynthesis proprietary resin as the separation matrix. This resin binds DNA under high salt concentrations and releases the bound DNA under low salt and slightly alkali conditions. The purified DNA can be used in a number of downstream applications including restriction enzyme digestions and ligations.

This Enzymatic Reaction Clean-Up Kit is able to purify DNA ranging in size from 100 bp to 15,000 bp. Each column has a binding capacity of 10 µg, and the typical purification yield is greater than 90% of the input amount. Each column can be used to purify up 100 µL of enzymatic reaction.

Features and Benefits:

• High Yield: Recovery is up to 90% of input.
• Versatile: Purify DNA from different enzymatic reactions including restriction enzyme digests, Klenow reactions, alkaline phosphatase reactions, and ligations.
• Fast: Rapid spin-column format allows for the processing of multiple samples in 15 minutes.
• Complete: Kits contain all columns and solutions required

Figure 1. Removal of Restriction Enzymes

This Enzymatic Reaction Clean-Up kit was used to remove the restriction enzyme HindIII from a digestion mixture. Lane A contains a digestion that was immediately stopped by the addition of EDTA, thus representing control DNA before column purification. Lane B represents the digested DNA, and serves as a positive control. Both lanes C and D contain digestion reaction that was immediately purified using Biosynthesis kit. In all cases, the DNA was incubated at 37°C for 1 hour after the specified treatment. It can clearly be seen that no digestion occurred in lanes C and D, and thus this Enzymatic Reaction Clean-Up Kit is able to successfully remove HindIII from a reaction mixture.

Figure 2. Removal of DNase I

This Enzymatic Reaction Clean-Up Kit was used to remove DNase I from a reaction. Lanes A to G of the gel contain DNA that was incubated with increasing amounts of DNase I for 1 hour, and thus act as control lanes for the digestion. Lanes A to G correspond to 1U, 0.5 U, 0.1 U, 0.05 U, 0.01 U, 0.005 U and 0.0001 U of DNase respectively. Lane H is a digestion of DNA with 0.5 U of DNase I that was immediately purified using Biosynthesis kit. By comparing lane H with the other control lanes, it can be determined that this Enzymatic Reaction Clean-Up Kit can remove DNase I to <0.0001>

For more detail regarding Peptide please visit here : Custom Peptide Synthesis