Catalog# : 3871
Bcl-2-associated athanogene 1 (BAG-1) was first identified as an anti-apoptotic bcl-2-binding protein. Later it was found to bind the molecular chaperones Hsp70 and Hsc70 through its carboxy-terminal sequence (termed the Bag domain), resulting in the inhibition of the refolding activity of these chaperones. It is thought that by binding and inhibiting these molecular chaperones, BAG-1is able to modulate the expression level of proteins requiring chaperones to fold correctly. One such group of proteins that are affected is glucocorticoid receptors. Other reports have suggested that the level of BAG-1 expression correlates with the aggressiveness of various cancers. Multiple isoforms of BAG-1 are known to exist.
Additional Names : BAG-1 (NT), Bcl-2-binding athanogene-1, receptor-associated protein 46, RAP46
DescriptionLeft: Western blot analysis of BAG-1 in PC-3 cell lysate with BAG-1 antibody at (A) 1 and (B) 2 µg/ml. Below: Immunohistochemistry of BAG-1 in human lymph node tissue with BAG-1 antibody at 2 µg/ml
Source : BAG-1 antibody was raised against a 14 amino acid peptide from near the amino terminus of human BAG-1.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : BAG-1 antibody was raised in rabbit
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat.No. 3871P - BAG-1 Peptide
Long-Term Storage : BAG-1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1216 - PC-3 Cell Lysate
- Cat. No. 1369 - Human Lymph Node Tissue Lysate
GI Number : 124494251
Accession Number : NP_004314
Short Description : (NT) Bcl-2, glucocorticoid receptor, Hsc70
References
- Takayama S, Sato T, Kraweski K, et al. Cloning and functional analysis of BAG-1: a novel Bcl2-binding protein with anti-cell death activity. Cell 1995; 80:279-84.
- Nollen EAA, Brunsting JF, Song J, et al. Bag1 functions in vivo as a negative regulator of Hsp70 chaperone activity. Mol. Cell. Biol. 2000; 20:1083-8.
- Cato ACB and Mink S. BAG-1 family of cochaperones in the modulation of nuclear receptor action. J. Steroid Biochem. & Mol. Biol. 2001; 78:379-88.
- Kajewska M, Turner BC, Shabaik A, et al. Expression of BAG-1 protein correlates with aggressive behavior of prostate cancers. Prostate 2006; 66:801-10.
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