Bcl-B Antibody

Bcl-B Antibody

Catalog# : 3163

Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-B is a recently discovered anti-apoptotic member of the Bcl-2. Unlike the mouse homolog (also known as Diva/Boo) which is predominantly expressed in ovary and testis, the human Bcl-B protein is widely expressed. Also, the human Bcl-B functions by binding to and suppressing the apoptotic activity of Bax, whereas the mouse homolog binds Bak and also interacts with the apoptosis protein Apaf-1. Despite its predicted molecular weight, Bcl-B is often at higher molecular weights, presumably due to post-translational modifications.

Additional Names : Bcl-B, Boo, Diva, Nrh

Description
Left: Western blot analysis of Bcl-B in Jurkat lysate with Bcl-B antibody at 1 µg/ml in the (A) absence and (B) presence of blocking peptide. Below: Immunocytochemistry of Bcl-B in Jurkat cells with Bcl-B antibody at 10 µg/ml.

Source : Bcl-B antibody was raised against a 16 amino acid peptide from near the amino terminus of human Bcl-B.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : Bcl-B antibody was raised in rabbit.

Tested Application(s) : E, WB, ICC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat.No. 3163P - Bcl-B Peptide

Long-Term Storage : Bcl-B antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control

• Cat. No. 1205 - Jurkat Cell Lysate

Species Reactivity :H

GI Number : 9966783

Accession Number : NP_065129

Short Description : an anti-apoptotic member of the Bcl-2 family

References

1. Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607
2. Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron. 2004; 39:1125-35.
3. Ke N, Godzik A, and Reed JC. Bcl-B: A novel Bcl-2 family member that differentially binds and regulates Bax and Bak. J. Biol. Chem. 2001; 276:12481-4.
4. Song Q, Kuang Y, Dixit VM, et al. Boo, a negative regulator of cell death, interacts with Apaf-1. EMBO J. 1999; 18:167-78.

For more detail regarding Peptides please visit here : Custom Peptide Synthesis