Apaf1 Antibody

Apaf1 Antibody

Catalog# : 2015

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologous of the key cell death gene CED-4 in C. elegans was identified recently from human and mouse and designated Apaf1 for apoptosis protease-activating factor 1 (1,2). Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (3). Apaf1 can also associate with caspase-4 and caspase-8 (4). Apaf1 transcript is ubiquitously expressed in human tissues .

Additional Names : Apaf1 (CT), Apaf1, Apaf-1

Description
Left: Western blot analysis of Apaf1 in human heart tissue lysate with Apaf1 antibody at 1 µg/ml dilution in the absence (A) or presence (B) of blocking peptide. Below: Immunohistochemistry of Apaf1 in human skin tissue with Apaf1 antibody at 20 µg/ml.

Source : Apaf1 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human Apaf1. The sequence of the immunogenic peptide differs from that of murine Apaf1 by one amino acid.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : Apaf1 antibody was raised in rabbit.

Immunogen : Human Apaf1 (C-Terminus) Peptide (Cat. No. 2015P)

Tested Application(s) : E, WB, IHC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 2015P - Apaf1 Peptide

Long-Term Storage : Apaf1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control

• Cat. No. 1301 - Human Heart Tissue Lysate

Species Reactivity : H, M, R

GI Number : 2330015

Accession Number : AAC51678

Short Description : (CT) Apoptosis protease activating factor

References

1. Zou H, Henzel WJ, Liu X, Lutschg A, Wang X. Apaf1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3. Cell 1997;90:405-13
2. Cecconi F, Alvarez-Bolado G, Meyer BI, Roth KA, Gruss P. Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development. Cell 1998;94:727-37
3. Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M, Alnemri ES, Wang X. Cytochrome c and dATP-dependent formation of Apaf1/caspase-9 complex initiates an apoptotic protease cascade. Cell 1997;91:479-89
4. Hu Y, Benedict MA, Wu D, Inohara N, Nunez G. Bcl-XL interacts with Apaf1 and inhibits Apaf1-dependent caspase-9 activation. Proc Natl Acad Sci USA 1998;95:4386-91 (WD1299)

For more detail regarding Peptides please visit here : Custom Peptide Synthesis