GFR alpha 3 Antibody

GFR alpha 3 Antibody

Catalog# : 1137

Members of the glial cell line-derived neurotrophic factor (GDNF) family including GDNF and neurturin (NTN) play essential roles in the control of vertebrate neuron survival and differentiation. A new member of the GDNF family was recently identified and designated persephin. Physiological responses to these neurotrophic factors require two receptor subunits, the novel glycosylphosphadidylinositol linked protein GFRalpha and Ret receptor tyrosine kinase GFRbeta (2). Following the findings of GFRΑ-1 and -2, a novel receptor in GFRalpha family was identified very recently from human and mouse and designated GFRalpha-3. GFRalpha-3 binds persephin, thus, persephin, GFRalpha-3, and Ret PTK form a complex to transduce persephin signal and to mediate persephin function.
Additional Names : GFR alpha 3 (IN), GFRa-3, GDNFRg, GDNFRgamma
Description
Left: Western blot analysis of GFR alpha 3 in crude cell membrane fractions of mouse heart (H), spleen (S), kidney (K), liver (L), and brain (B), respectively, with GFR alpha 3 antibody at 1:500 dilution.

Below: Immunohistochemistry of GFR alpha 3 in mouse heart tissue with GFR alpha 3 antibody at 10 µg/ml.

Other Product Images

Source : GFR alpha 3 antibody was raised against a peptide corresponding to amino acids from 347 to 360 of murine GFR alpha 3.
Purification : Antibody is DEAE purified
Clonality and Clone : This is a polyclonal antibody.
Host : GFR alpha 3 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Human GFR alpha 3 (Intermediate Domain) Peptide (Cat. No. 1137P)
Application : GFR alpha 3 antibody can be used for detection of GFR alpha 3 expression by Western blot at 1:500 to 1:1000 dilution.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 1137P - GFR alpha 3 Peptide
Long-Term Storage : GFR alpha 3 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1401 - Mouse Heart Tissue Lysate
2. Cat. No. 1404 - Mouse Liver Tissue Lysate
Species Reactivity :H, M, R
GI Number : 2627159
Accession Number : ab008833
Short Description : (IN) Persephin receptor
References

1. Milbrandt J, de Sauvage FJ, Fahrner TJ, et al. Persephin, a novel neurotrophic factor related to GDNF and neurturin. Neuron 1998;20:245-253.
2. Nomeclature Committee. Nomeclature of GPI-linked receptors for the GDNF ligand family. GFRa. Neuron 1997: 19:485.
3. Jing S. Yu Y, Fang M, Hu Z, Holst PL , Boone T, Delaney J. Schultz H, Zhou R, Fox GM. GFRalpha-2 and GFRalpha-3 are two new receptors for ligands of the DGNF family. J Biol Chem 1997; 272:33111-33117
4. Baloh RH, Gorodinsky A, Golden JP, Tansey MG, Keck CL, Popescu NC , Johnson EM Jr, Milbrandt J. GFRalpha3 is an orphan member of the GDNF/neurturin/persephin receptor family. Proc Natl Acad Sci USA 1998;95:5801-5806.