EVER1 Antibody

EVER1 Antibody

Catalog# : 4549

Epidermodysplasia verruciformis (EV) is an autosomal recessive dermatosis characterized by abnormal susceptibility to human papillomaviruses (HPVs) and a high rate of progression to squamous cell carcinoma on sun-exposed skin. EV is caused by mutations in either of two adjacent genes, EVER1 and EVER2, located on chromosome 17q25.3. Both of these genes encode integral membrane proteins that localize to the endoplasmic reticulum and are predicted to form transmembrane channels. Both EVER1 and EVER2 are members of the transmembrane channel-like (TMC) protein family. EVER1 possesses eight trans-membrane domains and two leucine zipper motifs. EVER1 and EVER2 form a complex and interact with the zinc transporter 1 (ZnT-1), suggesting that EVER1 and EVER2 act to regulate cellular zinc balance. At least four isoforms of EVER1 are known to exist. This EVER1 antibody does not cross-react with EVER2.
Additional Names : EVER1 (IN), EV1, EVIN1, Transmembrane protein channel-like protein 6, TMC6

Description
Left: Western blot analysis of EVER1 in human spleen tissue lysate with EVER1 antibody at (A) 1 and (B) 2 µg/ml.

Below: Immunohistochemistry of EVER1 in human spleen with EVER1 antibody at 2.5 µg/ml.

Other Product Images.

Source : EVER1 antibody was raised against a 15 amino acid peptide from near the center of human EVER1.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : EVER1 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : EVER1 antibody can be used for the detection of EVER1 by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat.No. 4549P - EVER1 Peptide
Long-Term Storage : EVER1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1306 - Human Spleen Tissue Lysate
Species Reactivity :H, M
GI Number : 25527208
Accession Number : AAM44452
Short Description : (IN) Transmembrane protein channel-like protein 6
References
1. Majewski S, Jablonska J and Orth G. Epidermodysplasia verruciformis. Immunological and nonimmunological surveillance mechanisms: role in tumor progression. Clin. Dermatol. 1997; 15:321-34.
2. Ramoz N, Rueda L-A, Bouadjar B, et al. Mutations in two adjacent novel genes are associated with epidermodysplasia verruciformis. Nat. Genetics 2002; 32:579-81.
3. Keresztes G, Mutai H and Heller S. TMC and EVER genes belong to a larger novel family, the TMC gene family encoding transmembrane proteins. BMC Genomics 2003; 4:24-34.
4. Lazarczyk L, Pons C, Mendoza JA, et al. Regulation of cellular zinc balance as a potential mechanism of EVER-mediated protection against pathogenesis by cutaneous oncogenic human papillomaviruses. J. Exp. Med. 2008; 205:35-42.